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Speech therapy for transgender women: an updated systematic review and meta-analysis

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We systematically reviewed the literature and performed a meta-analysis on the effects of speech therapy and phonosurgery, for transgender women, in relation to the fundamental frequency gain of the voice, regarding the type of vocal sample collected, and we compared the effectiveness of the treatments. In addition, the study design, year, country, types of techniques used, total therapy time, and vocal assessment protocols were analyzed.


We searched the PubMed, Lilacs, and SciELO databases for observational studies and clinical trials, published in English, Portuguese, or Spanish, between January 2010 and January 2023. The selection of studies was carried out according to Prisma 2020. The quality of selected studies was assessed using the Newcastle–Ottawa scale.


Of 493 studies, 31 were deemed potentially eligible and retrieved for full-text review and 16 were included in the systematic review and meta-analysis. Six studies performed speech therapy and ten studies phonosurgery. The speech therapy time did not influence the post-treatment gain in voice fundamental frequency (p = 0.6254). The type of sample collected significantly influenced the post-treatment voice frequency gain (p < 0.01). When the vocal sample was collected through vowel (p < 0.01) and reading (p < 0.01), the gain was significantly more heterogeneous between the different types of treatment. Phonosurgery is significantly more effective in terms of fundamental frequency gain compared to speech therapy alone, regardless of the type of sample collected (p < 0.01). The average gain of fundamental frequency after speech therapy, in the /a/ vowel sample, was 27 Hz, 39.05 Hz in reading, and 25.42 Hz in spontaneous speech. In phonosurgery, there was a gain of 71.68 Hz for the vowel /a/, 41.07 Hz in reading, and 39.09 Hz in spontaneous speech. The study with the highest gain (110 Hz) collected vowels, and the study with the lowest gain (15 Hz), spontaneous speech. The major of the included studies received a score between 4 and 8 on the Newcastle–Ottawa Scale.


The type of vocal sample collected influences the gain result of the fundamental frequency after treatment. Speech therapy and phonosurgery increased the fundamental frequency and improved female voice perception and vocal satisfaction. However, phonosurgery yielded a greater fundamental frequency gain in the different samples collected. The study protocol was registered at Prospero (CRD42017078446).

Peer Review reports


The voice is an important marker of gender identity; thus, many transgender women (TW) seek the speech therapy clinic for vocal improvement. Studies show that pitch is one of the main markers of gender in the voice of TW and that TW whose voices present a higher fundamental frequency (fo) are perceived as more feminine [1, 2]. Emerging evidence indicates that to be perceived as female, the voice foneeds to be at least between 145 and 165 Hz, called the “ambiguous pitch range.” Other aspects of human communication, both verbal and non-verbal, are important in the process of transition to a new gender identity [3].

Changes in vocal resonance can contribute to the perception of a female voice in TW because, in cis women, the frequencies of formants (Fm) are on average 20% higher than in men [4]. This fact is due to the result of anatomical differences (smaller resonance cavities in women) as well as behavioral differences in phonation (more retracted lips, in the form of a smile, and a more anterior tongue position). The importance of vocal resonance characteristics for gender identification is still not entirely clear.

Hormone therapy rarely results in the development of female vocal characteristics due to the irreversible changes in the laryngeal structure that occur during puberty. Thus, the treatment options for vocal feminization in TW are surgery and speech therapy—voice feminization therapy (VFT), alone or combined [3]. However, there is currently no standardized treatment protocol for vocal feminization.

Among several surgical procedures available for altering fo, those most cited in the literature are open techniques such as the cricothyroid approach and the modified laryngoplasty technique known as feminization laryngoplasty [5], which alter the laryngeal structure, and endoscopic approaches that focus on the vocal folds, such as Wendler glottoplasty and CO2laser techniques [3, 6]. These techniques are advised because the production of a female voice with a biologically male vocal organ carries a potential risk for vocal fatigue or trauma to the vocal folds, which would result in a perceptibly tense voice. Open surgical methods are based on three fundamentals: increasing tension, consistency, and decreasing the mass of the vocal folds [7]. However, surgery is not always sufficient to generate a female voice, and these procedures are not without complications [8]. One study found that phonosurgery carried significant risks of complications, such as reduced mean phonation time (61%), pitch instability (1.9%), decreased loudness (1.7 to 6%), vocal fatigue (6%), hoarseness (3%), and dysphonia (1.7%) [9].

Vocal complaints are common in the transgender, nonbinary, and gender-nonconforming (TNG) population [10]. For many TW, VFT is essential for the voice to be perceived as feminine. The focus of speech therapy is usually increasing fo; however, the satisfaction of individuals with their voices is not necessarily related to pitch. Other characteristics, such as intonation pattern, articulation, resonance, loudness, Fmpatterns, tongue placement in the oral cavity, airflow, pragmatics, and the way of speaking, are mentioned as important gender markers and are prescribed in VFT [3, 4].

When VFT is performed without the assistance of a specialized practitioner, the attempt to raise the pitch in a vocal apparatus with male anatomy can cause mild to moderate dysphonia or vocal tension. In addition, other aspects, such as vocal health, breathing exercises, and relaxation must be addressed, because TW is exposed to the same vocal risk behaviors of cisgender people, such as incorrect vocal use [3].

The benefits of VFT before and after surgery combined with the Wendler glottoplasty technique were reported in a study with 10 TW. All experienced significantly increased fo(mean increase 106 Hz), as well as significant improvements in the degree of voice feminization and self-reported satisfaction [11]. However, few studies describe the types of techniques used, their frequency and timing, the effects of these techniques on the fo, and voice quality outcomes, as well as which vocal techniques are most effective alone or in conjunction with vocal surgery.

Researchers report that VFT and behavioral changes are of great relevance in the TW voice transition process and that surgery can be indicated as an additional [3]. In addition, they suggest the need for rigorous studies to investigate the most effective methods for the vocal treatment of this population.

In a meta-analysis [9], concluded that both VFT and phonosurgery are efficient, depending on the individual need to increase fo, cost, and complications of the procedures. The authors did not analyze the types of vocal samples collected. Another study [12] report that the evidence for the effectiveness of VFT is still limited and that there is a lack of rigorous research to determine best-practice guidelines.

Authors [13] cited seven studies, before 2020, that provide empirical evidence of the effectiveness of voice training for TW, although still weak. Overall, voice training methods were similar but effective in increasing mean fo, fo range, vocal satisfaction, self-perception and listener perception of vocal femininity, voice-related quality of life, and social and professional participation. However, there is a lack of randomized controlled trials, small sample sizes, inadequate long-term follow-up, lack of control groups, and control of confounding variables. In addition, the last edition of the Standards of Care [13] recommends that health professionals who intend to work with transgender and gender-diverse people receive education to develop skills in supporting vocal functioning, communication, and well-being of this population; develop appropriate intervention plans for individuals dissatisfied with their voice and communication; and provide pre-and/or post-operative support.

Current studies [14, 15] provide more robust evidence of the effectiveness of speech therapy, despite the feminization of the voice for TW remains a challenge for professionals due to the lack of standardization of protocols, evaluation measurement, and effectiveness of the vocal techniques used. In a retrospective study [14] of 16 cases on the effects of a voice and communication modification program for TW, the results indicated that individuals showed a significant improvement in subjective results, even with small changes in acoustic measurements and vice versa. Another study [15] provided evidence that gender-affirming voice training for TW clients can be effective, both in the intensive and traditional form, in relation to acoustic measurements and vocal satisfaction; however, the training was not sufficient for all participants to reach their goal to develop a consistent feminine voice. Results from other research [16] agree with previous studies with continued targeting of fo and vocal tract resonance in voice and communication feminization/masculinization training programs, and provide preliminary evidence for more emphasis on vocal intensity and speech rate and for the importance of non-verbal communication targets in voice training programs and gender-affirming communication [17]. In addition, there are reports of the increase and effectiveness of online and/or hybrid care [18, 19].

Within this context, the underlying research questions of this systematic review were as follows: “What are the methods used in VFT for TW? What are the effects of VFT on the voice? What is the most effective approach (VFT or phonosurgery) concerning fo gain and type of sample voice?” Does the VFT time influence the fo gain?

Materials and methods

The ethical approval statement

This paper is part of the project “A Voz na Disforia de Gênero”, approved by the ethics committee of Hospital de Clínicas de Porto Alegre, Brazil, under number: 04075/2014. The study protocol was registered at PROSPERO (CRD42017078446).

Search strategy and study selection

In January 2023, two independent raters (KS and APVB) carried out a search of the PubMed, Lilacs, and SciELO databases for articles on the topic. The search strategy consisted of a combination of Medical Subject Headings (MeSH) descriptors and relevant keywords. The keywords used for the search were as follows: ((Transgender Persons) or (Health Services for Transgender Persons) or (Transsexualism) or (Gender Identity)) and ((Voice Training) or (Voice) or (Voice Quality)). The searches were adjusted to meet the requirements of each electronic database. The following filters used: studies published in the last 13 years (2010.01.01 to 2023.01.01).

According to PRISMA guidelines (2020) (Fig. 1), the selection of articles followed pre-established inclusion and exclusion criteria and a defined PICO question. Articles with the following characteristics were included as follows: design, observational studies (case–control and cohort) and clinical trials; participants (P), TW; intervention (I), VFT; control group (C), phonosurgery (VFTC) or no intervention; and outcome (O), fo and type of the sample voice.

Fig. 1
figure 1

Flow diagram of study selection

Data extraction and quality control assessment

The inclusion criterion was articles in Portuguese, English, or Spanish, from the last 13 years, and, according to the PICO questions, having the theme VFT or VFTC, in TW, which contained the result of the fo gain after the treatments and described the fo collection method.

The study exclusion criteria were as follows: (1) when individuals in the treatment groups did not undergo speech therapy, only surgery; (2) when not all individuals in the study group or control group underwent VFT; (3) when the study did not describe the gain of the fo of the voice post-treatment or when it did not mention the type of vocal sample collected; and (4) when the study sample was not composed only of TW.

The selection of studies consisted of four stages, with the following hierarchy of eligibility: (1) We removed the reviews and books, (2) duplicates were removed, (3) screening of articles according to the PICO question by reading the title and abstract, and (4) full-text reading by two study researchers (KS) and (APVB) and application of exclusion criteria. The flowchart in Fig. 1, according to PRISMA (2020) guidelines, shows the selection of study articles.

A descriptive analysis of the selected articles began with the application of a protocol prepared by the researchers, designed to collect the following data: authors, year of publication, country, study design, sample size and age, type of procedure, VFT methods, time-course variables of therapy, evaluations performed, results, means of measuring fo, pre- and post-fo data, and final fo gain, according to the type of the sample collected.

The methodological quality of the studies was assessed by three previously trained, independent reviewers using the Newcastle–Ottawa Scale (NOS) (KS, APVB, BG). The methodological quality score of the cohort and case–control studies was calculated in three components: selection of the groups (0–4 points), quality of adjustment for confounders (0–2 points), and evaluation of exposure after the outcome (0–3 points). The maximum score is 9 points, which represents high methodological quality.

Statistical analysis

The mean difference of fo gain, with a 95% confidence interval (CI), was estimated using a random effects model. We assessed heterogeneity between studies with I2 > 50% suggesting moderate heterogeneity and p < 0.10 on Cochran’s Q test indicating significant heterogeneity [20]. The following variables of interest were included in the meta-analysis: to compare the fo gain, in relation to the type of vocal sample collected (vowel, reading, or spontaneous speech), as well as the effectiveness between treatments, subgroup analysis was used. To evaluate the influence of treatment time (in sessions) concerning voice fo gain, meta-regression analysis was used. A p value of less than 0.05 was considered statistically significant. Statistical analyses were performed using R version 4.2.0 ( The meta package (version 5.2–0) for doing meta-analysis was used within the R environment.


Qualitative data synthesis

Characterization of the studies

Table 1 shows the year of publication of the studies included in the review and meta-analysis varied between 2012 and 2022, with a predominance of the year 2021, there is an exponential growth graph in recent years. Regarding the country of origin of the studies, the United States of America (USA) was the author who published the most on the subject.

Table 1 Description of the results about the treatments (VFT and phonosurgery)

Of the 16 studies included (Table 1) in the systematic review, six studies performed VFT [14, 15, 21,22,23,24] in the study group; among these, two carried out case–control studies, but the control group was without intervention. Others carried out cohort studies, retrospective and prospective, with and without a control group. The authors [15] compared two intervention groups with VFT (traditional and intensive) and investigated [24] the difference between VFT and phonosurgery (see Additional file 1).

As for phonosurgery, five studies were retrospective [25, 26, 30, 32] or prospective [31] cohorts and the other five were case-control [5, 11, 27,28,29] studies with a comparison of treatment results with different surgical techniques, with or without speech therapy, to groups of different ages (see Additional file 2).

Total therapy time

The total therapy time (Table 1) consisted of 5 to 84 sessions, one to three times a week, with an average duration of 1 h. Five studies [5, 26, 28,29,30] did not report the duration of therapy. In a study, patients received an average of 15 one-hour VFT sessions, twice a week; vocal changes persisted partially for 15 months after the end of therapy [23]. Another performed Wendler glottoplasty plus 24 post-surgery VFT sessions and reported a fogain of 106 Hz [25]. One study used intensive therapy (three 45-min sessions per week over 4 weeks) [15].

Speech therapy

Regarding VFT exercises, studies have recommended guidelines on phonotraumatic behaviors and vocal hygiene, relaxation techniques, and respiration training [21, 25]; Stemple Vocal Function Exercises [22, 23], with a focus on pitch, quality, intonation, and pitch range; laryngeal relaxation, cricothyroid dominant production exercises, resonance modification exercises, and exercises to form female Fm [26]. Some studies performed speech therapy only before surgery [30], and some did not mention the details of the techniques employed [27, 31, 32]. Information about the duration of speech therapy, as well as the techniques used and programs used for the collection of fodata, were lacking from many phonosurgery studies. Current studies recommend that speech therapy be focused on oral and nasal resonance, female speech patterns, improved voice efficiency, and body language [24]. In addition, the authors recommend exercises to maximize respiratory support with diaphragmatic breathing; head, neck, and torso stretch; vocal warm-up/semi-occluded vocal tract exercises; vocal function exercises; and resonant voice exercises, regular practice at home, and structured transfer tasks outside the clinic to facilitate generalization [15] and intonation strategies [14] (Table 1).

Vocal assessment

As for vocal assessment, some studies performed an acoustic voice analysis through a collection of the fo and administration of self-assessment questionnaires, with a predominance of the Transgender Woman Voice Questionnaire (TWVQ) [14, 24, 31, 32] and perceptual auditory analysis of voice using the GRBAS scale. The acoustic analysis programs differed between studies, with the multi-dimensional voice program by Kay Pentax being the most popular (Table 1).

Meta-analysis results

Gain of f o post-treatment and the type of vocal sample collected

There is a difference between the gain of fo and the type of vocal sample collected (Fig. 2).

Fig. 2
figure 2

Meta-analysis of the fo gain in the VFT, in relation to the types of samples collected. Legends: VFT, voice feminization therapy; VFTC, phonosurgery; VFT–I, voice feminization therapy – intensive; VFT–T, voice feminization therapy – traditional; VFTC–A, phonosurgery with group A; VFTC–B, phonosurgery with group B; VFT–G, voice feminization therapy plus glottoplasty; VFT–C, voice feminization therapy plus cricothyroid approximation; VFT–L, VFSRAC + LAVA—retrodisplacement of the anterior commissure (VFSRAC) associated with laser-assisted voice adjustment (LAVA) cordotomy

Figures 3, 4, and 5 show the greatest gain obtained in fo post-treatment refers to phonosurgery, that is, phonosurgery is the most effective treatment, independent of the collected sample (p < 0.01). As for the gain of fo, one study about anterior glottic web formation assisted by temporary injection augmentation [30] obtained the highest gain (110 Hz) and another [11] reported greater gain when speech therapy was combined with surgery (107 Hz) than with surgery alone (76 Hz) (see Additional file 2 and Fig. 2).

Fig. 3
figure 3

Comparison of the effectiveness of treatments (reading)

Fig. 4
figure 4

Comparison of the effectiveness of treatments (vowel /a/)

Fig. 5
figure 5

Comparison of the effectiveness of treatments (spontaneous speech)

Among the studies that performed phonosurgery (see Additional file 2), all reported a significant increase in post-treatment fo (p < 0.05), regardless of the type of vocal sample collected. The study [30] which as noted above obtained the highest gain (110 Hz), collected vowels, and the study with the lowest gain in fo 24 Hz in the control group), used spontaneous speech [31] (Fig. 2).

The main effects on the voice were increased fo and vocal satisfaction of the patient, improved quality of life, significant improvement of auditory-perceptual assessment, higher TMF, better self-perception of vocal quality, listener perception as a more feminine voice, and improvement of resonance.

Two studies [14, 15, 21,22,23,24] that evaluated the effects of VFT showed significant gains in fo, regardless of the type of sample collected (vowel, spontaneous speech, or reading), with less heterogeneity in gain between studies when collected with spontaneous speech (Figs. 2, 6, and 7). The highest gain of fowith VFT (55 Hz) was found in two studies [22, 23], in the reading sample, and the smallest gain utilizing the spontaneous speech sample (15 Hz) [24] (Fig. 2). A difference of 20 Hz was perceived in another study [21] (higher in vowels and lower in spontaneous speech) and 18 Hz (higher in reading and lower in spontaneous speech, vowel not collected) [23] (Fig. 2).

Fig. 6
figure 6

Gain of fo in phonosurgery, in relation to the type of vocal sample collected

Fig. 7
figure 7

Gain of fo in VFT, in relation to the type of vocal sample collected

VFT time and the f o gain

Figure 8 shows that the VFT time did not influence the fo gain result. Meta regression p = 0.6254, coeficient: − 0.1280 (CI 95% − 0.6420, 0.3859).

Fig. 8
figure 8

VFT time (in number of sessions) in relation to fo gain. Legends: test of moderators (coefficient 2): − 0.1280, (CI 95% − 0.6420, 0.3859); meta-regression p = 0.6254

Assessment of methodological quality

Regarding the assessment of methodological quality, Table 2 shows that most of the included studies received a score between 4 and 8 on the NOS. In brief, the studies’ sample sizes were small, there were selection biases regarding control groups, differences in variables of interest (vowel, spontaneous speech, and reading), and some studies did not mention the program used for acoustic analysis or the detailed description of the VFT method. There is a trend toward an increase in studies comparing different treatment methods, with well-defined groups and the same measurements of interest between groups.

Table 2 Newcastle-Ottawa Scale (NOS) for quality assessment of studies


This is the first systematic review to analyze the results of VFT and phonosurgery, stratified by different types of vocal samples collected (vowel, reading, or spontaneous speech), and report the evaluation protocols, instruments, and techniques used for the feminization of TW’s voices and the quality of selected studies by Newcastle–Ottawa Scale. Both VFT and phonosurgery resulted in a significant increase in the fo of the TW voice; however, phonosurgery provides a significantly greater gain. In both treatment approaches, the smallest gains occurred in the spontaneous speech sample, and even so, the individuals reached the frequency range where the voice could be perceived as female. In addition, the gain results are more homogeneous when spontaneous speech is used. These results provide important guidelines for clinicians who work with the transgender population since vocalizations with reading, automatic sequences, or vowels are situations in which TW can maintain greater self-control of vocal behavior than during spontaneous speech (Figs. 2, 3, 4, 5, 6, and 7).

In spontaneous speech, attention may be more focused on the message and interaction with the listener than on sustaining vowels, automatic speech, or reading; even after phonosurgery, several behavioral adjustments are necessary to ensure vocal adequacy. The focus on the message can divert attention from vocal production and justify the smaller gains in fo in a spontaneous speech reported by the different studies analyzed. Thus, it is suggested that isolated or post-surgical speech therapy focuses on prolonging or intensifying learning and the domains of vocal behaviors that facilitate the increase and maintenance of an fo closer to that obtained during automatic speech.

While studies of VFT were generally of small sample size and not randomized, the positive results in post-VFT fo gains, fovalues at follow-up, and perceptual analyses concerning femininity and masculinity corroborate the indication of VFT. One study notes that the literature supports both VFT and phonosurgery, depending on the magnitude of pitch gain desired by the client, costs, and possible complications. In addition, the authors concluded that VFT provides high vocal satisfaction to customers, is effective at increasing the pitch, and is not invasive [10]. Studies [14, 15, 24] that compared the effects of different methods of speech therapy, for TW, found that the tested methods are effective, with a significant improvement in auditory and acoustic voice perception, greater vocal satisfaction, greater congruence between gender identity and expression and reduction of negative impact on everyday life. Characteristics customers will look for surgery after vocal training are still unknown [13].

In this study, the relationship between therapy time (in sessions) and post-treatment fo gain was not significant (Fig. 8). Nonetheless, the authors [22] stated that both the largest number of sessions and the experience of living full-time as a woman can be important variables in predicting the progress of therapy. Another study [33] found that the largest change in fo was directly correlated with the increase in the number of vocal training sessions. One study with an average of 22 VFT sessions obtained an fo gain of 48 Hz and concluded that the only clear predictor for a result with a higher fois the increase in the number of treatment sessions [15]. Another study that reported the highest gain in fo (110 Hz), VFT was performed only before surgery, and the duration of therapy was not mentioned (Table 1) [30]. There is a need for further studies on the topic.

Regarding vocal assessment, the studies were uniform in terms of auditory-perceptual assessment through the application of the CAPE-V and GRBAS protocols (Table 1). In this sense, a previous study has shown that the two scales are reliable and indicated for analysis of voice quality; however, GRBAS was classified as the fastest and CAPE-V the most sensitive, mainly to detect small changes in voice, as in the case of TW [34].

Of note, in most of the included studies, acoustic analysis of the voice was performed by extraction of fo (in all studies) and measurement of the mean frequency range, perturbation, and noise (mean percentage jitter, shimmer, and noise-to-harmonics ratio). However, differences in study results and difficulties in comparing the analysis of VFT effects concerning those of phonosurgery can also be related to the various programs used in data extraction, forms of recording, environmental noise, or cultural factors that can affect amplitude and fo [35]. In addition, the gain was more homogeneous between studies when investigated in the spontaneous speech sample. There was a significant difference between the gain of the studies and the type of collection. In this way, it is suggested a greater standardization in the collection of pre and post-treatment fo, to compare results and the use of spontaneous speech in the sample collection, as it better translates the patient’s speech and contributes to the generalization of the highest fo gain to spontaneous speech (Fig. 2).

In a study that set out to identify which are the predictive parameters of masculinity-femininity ratings by presenting auditory and visual cues for transgender and cisgender, the authors found statistical significance to fo, average vowel Fm, and sound pressure level [17]. Indeed, a study [36] report that methods for voice feminization are based on changing four parameters: fo, resonance frequency relative to vocal tract volume and length, Fm tuning, and phonatory pattern. In the present systematic review, only two studies that analyzed the first three Fm of /i/ from the word “beach”, the first three Fm for /i/, /a/, and /u/ (from the isolated vowels); and /i/ from the selected semi-spontaneous Q/A set included Fmanalysis [22, 23]. One study showed that the frequencies of forming vowels are important clues to the perception of gender, mainly in the range between 145 and 165 Hz, and more prominent in spontaneous speech than in isolated vowels or syllables [37]. A research [26] used resonance modification exercises to form female Fm based on the source/filter theory of voice production. Thus, it is important to include an investigation of Fm through spectrographic vocal analysis in future protocols for voice evaluation in TW.

The most used self-assessment questionnaire among the included studies was the Trans Woman Voice Questionnaire (TWVQ) followed by the Transgender Self-Evaluation Questionnaire (TSEQ) and Voice Handicap Index (VHI) (Table 1). The TSEQ is the oldest questionnaire developed for this purpose [38, 39] and was modified from the VHI for the transgender population. Although TSEQ is widely used by researchers in North America and abroad, there are few studies on its characteristics, and its psychometric properties have not yet been established. Scientific evidence supports the validity and indication of the TWVQ as an important tool for TW to perceive the functioning of their voices and how it impacts their daily lives [40].

Both studies focusing on VFT [21, 23] and those combining it with phonosurgery [11, 25] recommend guidelines on phonotraumatic behaviors vocal hygiene, relaxation techniques, and respiration training, because transgender people are exposed to the same types of vocal misuse behaviors of cisgender people. Some phonosurgery studies did not mention the details of the VFT exercises and recommendations before or after surgery, which makes it difficult to compare the effects of VFT on the voice in relation to phonosurgery.

The Stemple Vocal Function Exercises (VFE) method was used in two studies [22, 23]. Stemple’s physiological vocal therapy is based on anatomy and physiology and seeks to modify the function of the laryngeal musculature and the respiratory support provided for voice production. The approach involves direct modification of inappropriate physiological activity through exercises, which focus on airflow and strength of the laryngeal muscles to balance the breathing, phonation, and resonance systems. A study [23] recommends that the main objective of VFT is the modification of pitch, quality, intonation, and pitch range and started their sessions with the production of the consonant /m/ plus vowels with the new fo target for habituation and facilitation of oral resonance. A target fo was chosen for each person based on age, vocal range, and initial fo measurements. Afterward, words starting with /n/, /l/, and /r/ were introduced, followed by phrases with the objective of training intonation, pitch, and vocal quality, and, finally, multiple sentences involving descriptions of figures, roleplaying, and open-ended questions. Two studies [22, 23] also recommend the use of the Real-Time Pitch Software from Multi-Speech for immediate feedback on the appropriate frequency and production of smooth voice quality (Table 1).

Among the six studies that performed VFT, three used intonation as one of the focuses of therapy. The authors showed that intonation was the target of VFT in 68% of cases; during VFT, the client should demonstrate an appropriate vocal variety and increase in rising intonation, regardless of the objective of increasing fo, and practicing with phrases, sentences, and paragraphs [21]. Research is still inconclusive about the use of intonation [41], but it appears that most authors who researched VFT recommend its use. Among the phonosurgery studies, there was no such recommendation, but many studies did not detail the method used in speech therapy (Table 1).

As for resonance exercises, the three studies of VFT and two of phonosurgery recommended this modality. The authors [23] reported that 96% of clients used resonant voice in words with initial-position bilabial and semivowel phonemes, with resonance exercises involving nasal sounds and auditory feedback [21]. Most studies recommend moving resonance forward into the oral cavity (“lip spreading and forward tongue carriage”) as a focus of therapy [11, 23, 29], initially in isolated vowels, consonant–vowel, and vowel-consonant syllables, followed by monosyllabic words, bisyllabic words, phrases, full sentences, and conversation. The other studies did not specify how vocal resonance was targeted.

The results of one prospective study found that the TW voice was perceived as female for vowels that had increased frequencies in the Fm, in addition to an increase in fo [42]. In a study [4] on the effectiveness of five oral resonance therapy sessions with 10 TW, targeting lip spreading and forward tongue carriage, showed that both the values of the first Fm (F1, F2, and F3) of vowels /a/, /i/, and // and fo increased after VFT, with the change in F3being statistically significant. In addition, most TW were perceived as more feminine by others and through self-assessments and were more satisfied with their voices [4]. Oral resonance therapy appears promising and is consistent with the fact that Fmdiffers between genders; however, further research is needed to prove the effectiveness of the method for TW. One study recommended the use of vocabulary, pragmatic [28], and two nonverbal communication for feminization [24]. There is no scientific evidence of gender differences in these parameters [28].

As for surgical techniques combined with VFT, Wendler glottoplasty was the most cited, in seven studies with an fo gain of up to 107 Hz [11] (see Additional file 2 and Fig. 1). The study [30] obtained the greatest gain in fo (110 Hz) with pre-surgery VFT and anterior web formation with injection augmentation. The lowest gain of fo was collected through spontaneous speech, using VFT (15 Hz) [24]. A reserach [11] studying the effect of phonosurgery in relation to surgery alone showed that VFT promotes better gain in fo when combined with surgery (107 Hz with VFT vs. 76 Hz without VFT), corroborating the results of this study, which showed that phonosurgery is more effective than speech therapy alone, in relation to the gain in fo. One research reported that endoscopic shortening was more effective in raising fo, with an increase of more than 70 Hz, versus a change from 26 to 40 Hz achieved by VFT and other surgical options [9].

The authors [3] state that the variations between these studies are mainly due to the complex nature of the individual, differences in vocal demands, and the lack of standardization of vocal feminization treatments among voice practitioners. In addition, the assessment of methodological quality using the NOS showed great variability between studies in how fo was collected, which acoustic analysis software was used; sample sizes are small, and there are biases in the selection of control groups, as well as the lack of detailed information about the speech therapy steps and techniques used. This made it difficult to compare results across the included studies.

The limitations of the present review are the small number of published articles, their small sample sizes, and the differences in the fo analysis methods of each study. As we verified differences between the different types of collected voice samples, it is not possible to group all the results without considering these differences.


Both VFT and phonosurgery showed an increase in the fo of the voice, regardless of the type of vocal sample collected, with phonosurgery having presented a significative greater gain of fo. The type of vocal sample collected significantly influenced the result of fo gain after treatment. The speech therapy time did not influence the post-treatment gain in voice fo. The quality of evidence of the studies was low, given the lack of randomized controlled trials, the small sample sizes, and the different methods of collecting fo. Thus, this study may provide additional evidence on the role of VFT and phonosurgery on voice feminization and encourage further research in the field.

Availability of data and materials

We have one supplementary material.

Change history

  • 10 August 2023

    An article "A" has been inadvertently added at the beginning of the title. The article has been updated to rectify the errors.


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The authors would like to acknowledge the contributions of the speech therapist Maria Elza Dorfman for the availability and care of transgender individuals at the Hospital de Clínicas de Porto Alegre. We also thank the research funders: Fundo de Incentivo a Pesquisa e Eventos do Hospital de Clínicas de Porto Alegre (FIPE/HCPA), Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (grant number INCT/FAPERGS: 17/2551-0000519-8), National Council for Scientific and Technological Development (CNPq), Coordination for the Improvement of Higher Education (CAPES), and Pos Graduate Program in Behavioral Sciences, Psychiatry at UFRGS. C. A. Cielo acknowledges support from the Brazilian agency CNPq (Grant 305151/2022-3).


Fundo de Incentivo a Pesquisa e Eventos do Hospital de Clínicas de Porto Alegre,Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul,17/2551-0000519-8,Karine Schwarz,National Council for Scientific and Technological Development,305151/2022-3),Carla Aparecida Cielo,Coordination for the Improvement of Higher Education (CAPES),Pos Graduate Program in Behavioral Sciences,Psychiatry at UFRGS.

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Authors and Affiliations



Conceptualization: KS and PMS. Data curation: KS, APVB, DCS, and BG. Formal analysis: KS and PMS. Funding acquisition: PMS and ABC. Investigation: MIRL. Methodology: KS, PMS,and MAS. Project administration: MIRL. Software: AMVF. Supervision: MIRL and CAC. Validation: KS and PMS. Visualization: KS and APVB. Writing: BG, KS, PMS, and CAC.

Corresponding author

Correspondence to Poli Mara Spritzer.

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The work was performed at the Department of Psychiatry, Gender Identity, Program at Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil

Supplementary Information

Additional file 1.

Effects of voice feminization therapy on fo in transgender women Legends: NA: not available; SD: standard deviation; fo: fundamental frequency of voice. * Statistical result found by the authors of the study cited in the comparison of fo gain between the pre and post treatment or Significant effects for therapy time versus fo significant effects for time versus fo 15.

Additional file 2.

Effects of phonosurgery on fo in transgender women Legends: SD: standard deviation; fo: fundamental frequency; * Statistical result found by the authors of the study cited in the comparison of f0 gain between the pre and post treatment. * Wendler glottoplasty (WG) and its modification, the vocal fold shortening, and retrodisplacement of the anterior commissure (VFSRAC) associated with laser assisted voice adjustment (LAVA) cordotomy. VFT: Voice Feminization Therapy.

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Schwarz, K., Cielo, C.A., Spritzer, P.M. et al. Speech therapy for transgender women: an updated systematic review and meta-analysis. Syst Rev 12, 128 (2023).

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