Skip to main content

Protocol for a systematic review of outcomes from microsurgical free-tissue transfer performed on short-term collaborative surgical trips in low-income and middle-income countries



In many units around the world, microsurgical free-tissue transfer represents the gold standard for reconstruction of significant soft tissue defects following cancer, trauma or infection. However, many reconstructive units in low-income and middle-income countries (LMICs) do not yet have access to the resources, infrastructure or training required to perform any microsurgical procedures. Long-term international collaborations have been formed with annual short-term reconstructive missions conducting microsurgery. In the first instance, these provide reconstructive surgery to those who need it. In the longer-term, they offer an opportunity for teaching and the development of sustainable local services.


A PRISMA-compliant systematic review and meta-analysis will be performed. A comprehensive, predetermined search strategy will be applied to the MEDLINE and Embase electronic databases from inception to August 2021. All clinical studies presenting sufficient data on free-tissue transfer performed on short-term collaborative surgical trips (STCSTs) in LMICs will be eligible for inclusion. The primary outcomes are rate of free flap failure, rate of emergency return to theatre for free flap salvage and successful salvage rate. The secondary outcomes include postoperative complications, cost effectiveness, impact on training, burden of disease, legacy and any functional or patient reported outcome measures. Screening of studies, data extraction and assessments of study quality and bias will be conducted by two authors. Individual study quality will be assessed according to the Oxford Evidence-based Medicine Scales of Evidence 2, and risk of bias using either the ‘Revised Cochrane risk of bias tool for randomized trials’ (Rob2), the ‘Risk of bias in non-randomized studies of interventions’ (ROBINS-I) tool, or the National Institute for Health Quality Assessment tool for Case Series. Overall strength of evidence will be assessed according to the Grading of Recommendations, Assessment, Development and Evaluations (GRADE) approach.


To-date the outcomes of microsurgical procedures performed on STCSTs to LMICs are largely unknown. Improved education, funding and allocation of resources are needed to support surgeons in LMICs to perform free-tissue transfer. STCSTs provide a vehicle for sustainable collaboration and training. Disseminating microsurgical skills could improve the care received by patients living with reconstructive pathology in LMICs, but this is poorly established. This study sets out a robust protocol for a systematic review designed to critically analyse outcomes.

Systematic review registration


Peer Review reports


Five billion people around the world are living without access to adequate surgical care [1]. Short-term collaborative surgical trips (STCSTs) provide an opportunity for surgical teams from higher income nations to collaborate with surgeons from low-income and middle-income countries (LMICs) to treat those most in need. Historically, some STCSTs have been criticised for an unsustainable ‘fly-in-fly-out’ model of surgical care delivery in LMICs, with limited patient follow-up after discharge. One consequence of this is that surgeons working in resource-limited settings have a poor context-specific evidence base to work from [2]. However, many STCSTs return annually to the same centre, building lasting relationships, offering sustainable education and training and treating patients who would otherwise have no access to healthcare. This model offers greater opportunity for thorough follow-up. Indeed, new methods of long-term patient follow-up after discharge are emerging that aim to improve patient safety after STCSTs in LMICs [3, 4]. Historically, such trips were often referred to as ‘missions’. However, there is a growing consensus that the model described above is better captured by the term ‘collaboration’ [5].

Free-tissue transfer can be considered the gold-standard method of reconstruction after significant composite tissue defects from cancer, trauma and infections [6,7,8,9]. In the largest series from high volume centres, flap failure rates as low as 0.6%, and take back rates of 1.5% (66% successful salvage rate) have been published [10]. For surgeons operating in LMICs, that do not have funding and access to the required equipment and training to facilitate independent microsurgical practice, treatment options are restricted to those used 50 years ago in countries that now perform regular free-tissue transfer. For select advanced pathology, this may be the difference between amputation and lower limb salvage after trauma and sarcoma resection, or poorer functional outcomes following head and neck cancer reconstruction [11,12,13,14,15]. Performing microsurgery in LMICs is challenging, even for experienced teams. However, a select few centres in LMICs are undertaking these procedures as part of standard practice [16,17,18]. Local and regional reconstructive alternatives do exist (e.g. transposition or pedicled flaps); however, many of these are also technically demanding, and even with extensive training, microsurgical equipment and techniques, they are vulnerable to complications requiring further surgery [19,20,21,22]. However, there is also some data in support of successful local reconstructive alternatives being used effectively in low-resource contexts [22].

There are existing reviews of STCSTs, but none provide the reader with a detailed account of microsurgical free-tissue transfer performed on this basis, and many combine surgical trips with medical ones in their analysis. There have been studies on socioeconomic/political impact, cost-effectiveness, sustainability and quality of follow-up [2, 23,24,25]. However, it has been difficult to draw any firm conclusions due to the paucity of high-quality quantitative data, and the heterogeneity of these interventions, which are often organised on a departmental basis [2]. The closest review to our proposed study assessed a number of outcomes from ‘short-term reconstructive missions’, although the majority of patients in their analysis underwent cleft surgery (without microsurgical free tissue transfer) [26]. They cite substantial reporting bias when it comes to operative complications, and highly variable estimates of cost effectiveness for the same intervention depending on local context.

There are some fundamental challenges to providing microsurgery in LMICs. Amongst those reported are a lack of specialist equipment, trained staff and appropriate level three post-operative monitoring facilities [27]. STCSTs, which can provide additional staff and resources, can directly address these challenges. However, these are usually short-term interventions, and patients may not be afforded the same degree of follow-up provided by the home institutions of the visiting surgeons. In addition, it is traditionally held that reconstructive surgeons should stick to ‘simple’ surgery on STCSTs [28]. Therefore, it is important to establish the safety of delivering free-tissue transfer on STCSTs. Identification of common complications and constraints will inform organisations engaged in STCSTs where to focus their training and fundraising efforts in order to improve patient outcomes. The highly specialised nature of this surgery means that it is difficult to draw on the existing review articles in the literature to make any firm conclusions about this technique performed during STCSTs.

The aim of this study is to publish a robust systematic review protocol to establish the safety and efficacy of microsurgical free tissue transfer performed on STCSTs in low-income and middle-income countries. A meta-analysis of key outcomes will be undertaken with the aim of developing potentially life-changing microsurgical practice in resource-limited settings.


This protocol is registered on the PROSPERO international prospective register of systematic reviews (submitted 15/12/20 ID: 225613) and is written in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analysis Protocols (PRISMA-P) guidelines [29, 30]. The methodology applied to the final systematic review and meta-analysis is derived from, and in line with, the Cochrane Handbook for Systematic Review of Interventions [31] and the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement [32].

Search strategy

The search strategy (Table 1) will be applied to the MEDLINE and Ovid EMBASE databases from inception to 5 August 2021.

Table 1 Electronic database search—MEDLINE and Ovid EMBASE

Study selection criteria

Inclusion criteria

All clinical studies in the English language reporting outcomes of any microsurgical procedure performed on a short-term reconstructive trip to any low-income or middle-income country (in accordance with the World Bank Classification [33]) will be eligible for inclusion. Studies that match the inclusion criteria performed in low-resource environments in a high-income country will also be included. Children and adults will be considered. All cases performed using either operating microscope or loupe magnification will be included, as both are successfully reported in resource-limited settings [16]. The Population, Intervention, Comparison, Outcome (PICO) model was used to determine study selection criteria (Table 2) [34].

Table 2 Population, Intervention, Comparison, Outcome (PICO)

Exclusion criteria

Studies that do not provide sufficient data for comparative analysis will be excluded. Where incomplete or absent data is presented in a given study, study authors will be contacted by email on a maximum of two separate occasions, 2 weeks apart, inviting them to provide further data before being excluded. Data presented from microsurgical units already independently performing microsurgery in low-income or middle-income countries, not on a STCST, will also be excluded.

Study design

Randomised controlled trials (RCTs), cohort, case-control and case series will be considered for inclusion. Pilot searches indicate that the majority of studies will be case series of varying sizes. As such, no limitation regarding study size or clinical follow-up will be made. Case reports, letters, opinion pieces and literature reviews will be excluded. Any unpublished or ongoing prospective clinical trials will also be excluded.


The participants are children or adults that have undergone microsurgical free-tissue transfer during a short-term collaborative surgical trip to a low-income or middle-income country. No limitations based on patient demographics, body region or aetiology will be imposed.


Primary outcomes

The primary outcomes are rate of free-flap failure and rate of emergency return to theatre for attempted free-flap salvage. Return to theatre will be classified as a donor site or anastomotic complication. The time taken to return to theatre, and the rate of successful flap salvage will also be documented where available. Where disclosed, we will record the method of flap salvage attempted.

Secondary outcomes

The secondary outcomes will include complications and any functional or patient-reported outcome measures included. Based on previous large case studies of free flaps, complications will be divided into medical and surgical. The surgical group will be further subdivided into intraoperative and post-operative. Complications will be stratified according to the Clavien-Dindo classification [35,36,37]. Finally, if available any assessment of pre-operative fitness will be recorded.

Additional data

In addition to the primary and secondary outcomes, duration of procedure and length of stay will also be recorded where available. In the context of a STCST to a LMIC, this is particularly important. The following will also be extracted: bibliographic data (title, author, date), study characteristics (design, method of randomisation/allocation, blinding, number of participants, groups/subgroups), mission characteristics (country, length of mission, type of mission/subspecialty, organisation (type and size), type of hospital (e.g. public or private), frequency of missions), patient characteristics (age, sex, indication for surgery, comorbidities, smoking status), intervention characteristics (operation(s) performed, duration of operation, length of stay, who performed the surgery (local or visiting surgeon), experience level of surgeon who performed anastomosis and raised flap, pre-operative workup), and rate and duration of follow-up.

Outcomes will be compared to data available from the multicentre, UK National Flap Registry, published in 2019 [9].

Data management and extraction

Abstracts will be screened on the Rayyan systematic review software tool. Full papers will be downloaded as PDFs, and stored locally on Mendeley Desktop. All abstracts included will proceed to full-text analysis unless it is immediately apparent following reading of the introduction that they are irrelevant. Data items will be collected in a standardised data collection proforma. For instances of incomplete data, we will contact the corresponding author. If 2 weeks elapse with no response, we will repeat this request once.

Data selection

Screening will be conducted and recorded in accordance with the Preferred Reporting Items for Systematic Review and Meta-Analysis (PRISMA) guidelines [32]. Abstracts screened according to criteria set out in this protocol by two independent researchers (HdB and UC). In case of disagreements, researchers will meet to discuss disparities; if there are still disagreements, a third author (CH) will make the final decision on inclusion. If abstracts are not available on assessment, the paper will be downloaded in full for analysis.

Risk of bias assessment

Each study will be assessed for risk of bias by two independent reviewers (HdB and UC) according to an appropriate validated tool. Randomised studies will be assessed using the revised Cochrane risk of bias tool for randomised trials (Rob2) [38]. Non-randomised studies will be assessed using the ‘Risk of bias in non-randomised studies of interventions’ (ROBINS-I) tool [39]. Case series will be assessed using the National Institute of Health Quality Assessment Tool for Case Series Studies [40].

Quality of studies

Each study will be assessed according to the Oxford Evidence-based Medicine Scales of Evidence 2 [41]. This data will be tabulated.

Strategy for data analysis and synthesis

Statistical analysis of included studies will be undertaken in R version 4.0.5 (R Foundation for Statistical Computing, Vienna, Austria). Patient demographics will be presented using basic descriptive statistics. Complications, free flap failure, emergency return to theatre and successful free-flap salvage will be calculated and displayed as rate (%). Data from the first UK National flap registry will be used as a comparator [9]. Statistical heterogeneity will be examined by calculating I2 and Cochran’s Q statistic, and interpreted according to Cochrane guidance on determining heterogeneity [31]. If I2 > 50, a random-effects model (DerSimonian and Laird with a logit transformation applied) will be used to calculate relative risk with 95% confidence intervals [42]. If I2 ≤ 50, a fixed-effects model will be used for relative risk calculations. A p value of < 0.05 will be considered statistically significant. The results of this meta-analysis will be presented in Forest plots. Funnel plots will be used to detect publication bias. If quantitative analysis is inappropriate, a narrative synthesis will be performed.

The analysis detailed above will be undertaken for the pooled data. If sufficient data is available, subgroup analysis by region (e.g. head and neck, trunk etc.) will be undertaken.

Confidence in cumulative evidence

The body of evidence underpinning each of the findings will be assessed according to the ‘Grading of Recommendations, Assessment, Development and Evaluations’ (GRADE) approach [43]. Using this approach, the authors will express their ‘certainty’ that the body of evidence reflects reality (high, moderate, low, very low).


This will be the first systematic review and meta-analysis of the outcomes of free-tissue transfer performed on STCSTs to low-income and middle-income countries. Our pilot searches indicate that the majority of data will be published in case series’; it is difficult to form a conclusion from any one of these alone. This underlines the importance of a thorough synthesis of these studies into a unifying review article. However, lack of randomised allocation and control groups increase the risk of bias, and this has led to concerns regarding the quality of evidence in this field [26]. We cannot remedy this, but will provide the reader with an open appraisal of the body of evidence according to the GRADE approach. From this, they will be able to form their own conclusions about their confidence in our findings.

We predict considerable clinical heterogeneity between the studies included in this paper. STCSTs are often based on relationships between specific institutions, sometimes individual surgeons, and thus there is no ‘one size fits all approach’. Another source of heterogeneity will come from the inclusion of all flap types. We will try to mitigate this through sub-group analysis if possible, but there may be insufficient data available.

Nutritional status has been identified as a determinant of free flap outcome [44]. STCSTs treat a diverse population of patients, many of whom will have poor nutritional status. This may be a confounding factor in reconstructive outcomes, but is unlikely to be well documented in a standardised manner across the literature. We will collect nutritional and other preoperative assessments where possible, but there may not be sufficient data to adjust for these variables.

We hope to equip surgeons undertaking STCSTs with the relevant data to offer appropriate treatment to their patients in order to achieve the best possible outcomes. In addition, this will ensure patients are able to give informed consent with an understanding of the risks specific to their situation. These impacts will improve patient safety, reconstructive outcomes and follow-up.

Finally, with appropriately strict governance surrounding distribution of funds designed to provide healthcare in low- and middle-income countries, it is essential that actors in this field are able to provide evidence supporting their work. Through identifying the nature and severity of complications, we hope to inform surgeons and funders of the challenges and barriers to free tissue transfer in LMICs. This should encourage investment in areas that are most likely to improve patient outcomes. This study will assist those funding global surgery to allocate resources to appropriate interventions with proven patient benefit.

Availability of data and materials

Data sharing is not applicable to this article as no datasets were generated or analysed during the current study.



Elsevier Biomedical and Pharmacological Bibliographic Database


Grading of Recommendations, Assessment, Development and Evaluations


Low- and middle-income countries


US National Library of Medicine Bibliographic Database


Medical Subject Headings


Preferred Reporting Items for Systematic Reviews and Meta-Analyses


International Prospective Register of Systematic Reviews


Randomised controlled trial


Revised Cochrane risk of bias tool for randomised trials


Risk of bias in non-randomised studies of interventions


Short-term collaborative surgical trip


  1. 1.

    Meara JG, Leather AJM, Hagander L, Alkire BC, Alonso N, Ameh EA, et al. Global surgery 2030: evidence and solutions for achieving health, welfare, and economic development. Lancet. 2015;386(9993):569–624.

    Article  PubMed  Google Scholar 

  2. 2.

    Shrime MG, Sleemi A, Ravilla TD. Charitable platforms in global surgery: a systematic review of their effectiveness, cost-effectiveness, sustainability, and role training. World J Surg. 2015;39(1):10–20.

    Article  PubMed  Google Scholar 

  3. 3.

    Bradley D, Honeyman C, Patel V, Zeolla J, Lester L, Eshete M, Demissie Y, Martin D, McGurk M. Smartphones can be used for patient follow-up after a surgical mission treating complex head and neck disfigurement in Ethiopia: Results from a prospective pilot study. J Plast Reconstr Aesthet Surg. 2021;74(4):890–930.

  4. 4.

    Honeyman C, Patel V, Yonis E, Fell M, Demissie Y, Eshete M, et al. Long-term outcomes associated with short-term surgical missions treating complex head and neck disfigurement in Ethiopia: a retrospective cohort study. J Plast Reconstr Aesthet Surg. 2020;73(5):951–8.

    CAS  Article  PubMed  Google Scholar 

  5. 5.

    Jemec B, Lam W, Hodgson SP, Jones JWM, Eckersley R, Nyamulani N, et al. The governance of overseas surgical collaborations – BFIRST/BSSH. J Plast Reconstr Aesthet Surg. 2021;74(2):396–400.

    CAS  Article  PubMed  Google Scholar 

  6. 6.

    Shah SC, Kayamba V, Peek RM, Heimburger D. Cancer control in low- and middle-income countries: is it time to consider screening? J Glob Oncol. 2019;5:1–8.

    CAS  Article  PubMed  Google Scholar 

  7. 7.

    Semer NB, Sullivan SR, Meara JG. Plastic surgery and global health: how plastic surgery impacts the global burden of surgical disease. J Plast Reconstr Aesthet Surg. 2010;63(8):1244–8.

    Article  PubMed  Google Scholar 

  8. 8.

    The World Health Organisation. The World Health Organisation. Injuries and violence: the facts [Internet]. 2010. Available from: Accessed 12 Dec 2020.

  9. 9.

    Hazari A, Cole R, Fowler C, Schache A, Nugent M, Ho M, et al. The British Association of Plastic Reconstructive and Aesthetic Surgeons First UK National Flap Registry Report. 2019.

    Google Scholar 

  10. 10.

    Carney MJ, Weissler JM, Tecce MG, Mirzabeigi MN, Wes AM, Koltz PF, et al. 5000 free flaps and counting. Plast Reconstr Surg. 2018;141(4):855–63.

    CAS  Article  PubMed  Google Scholar 

  11. 11.

    Lewin J, Puri A, Quek R, Ngan R, Alcasabas AP, Wood D, et al. Management of sarcoma in the Asia-Pacific region: resource-stratified guidelines. Lancet Oncol. 2013;14(12):e562–70.

    Article  Google Scholar 

  12. 12.

    Ducic I, Rao S, Attinger C. Outcomes of microvascular reconstruction of single-vessel lower extremities: limb salvage versus amputation. J Reconstr Microsurg. 2009;25(08):475–8.

    Article  PubMed  Google Scholar 

  13. 13.

    Millard DR, Dembrow V, Shocket E, Zavertnik J, Clinton-Thomas C. Immediate reconstruction of the resected mandibular arch. Am J Surg. 1967;114(4):605–13.

    CAS  Article  PubMed  Google Scholar 

  14. 14.

    Habib AMA, Hassan SA. The feasibility of rib grafts in long span mandibular defects reconstruction: a long term follow up. J Cranio-Maxillofacial Surg. 2019;47(1):15–22.

    Article  Google Scholar 

  15. 15.

    Lou C, Yang X, Hu L, Hu Y, SP, Loh J, et al. Oromandibular reconstruction using microvascularized bone flap: report of 1038 cases from a single institution. Int J Oral Maxillofac Surg. 2019;48(8):1001–8.

    CAS  Article  PubMed  Google Scholar 

  16. 16.

    Nangole WF, Khainga S, Aswani J, Kahoro L, Vilembwa A. Free flaps in a resource constrained environment: a five-year experience—outcomes and lessons learned. Plast Surg Int. 2015;2015(September):1–6.

    Article  Google Scholar 

  17. 17.

    Nakarmi KK, Rochlin DH, Basnet SJ, Shakya P, Karki B, Magar MG, et al. Review of the first 108 free flaps at Public Health Concern Trust-Nepal Hospitals: challenges and opportunities in developing countries. Ann Plast Surg. 2018;81(5):565–70.

    CAS  Article  PubMed  Google Scholar 

  18. 18.

    Trivedi NP, Trivedi P, Trivedi H, Trivedi S, Trivedi N. Microvascular free flap reconstruction for head and neck cancer in a resource-constrained environment in rural India. Indian J Plast Surg. 2013;46(1):82–6.

  19. 19.

    Schaverien MV, Hamilton SA, Fairburn N, Rao P, Quaba AA. Lower limb reconstruction using the islanded posterior tibial artery perforator flap. Plast Reconstr Surg. 2010;125(6):1735–43.

    CAS  Article  PubMed  Google Scholar 

  20. 20.

    Wong JKF, Deek N, Hsu C-C, Chen H-Y, Lin C-H, Lin C-H. Versatility and “flap efficiency” of pedicled perforator flaps in lower extremity reconstruction. J Plast Reconstr Aesthet Surg. 2017;70(1):67–77.

    Article  PubMed  Google Scholar 

  21. 21.

    Olawoye OA, Ademola SA, Iyun K, Michael A, Oluwatosin O. The reverse sural artery flap for the reconstruction of distal third of the leg and foot. Int Wound J. 2014;11(2):210–4.

    Article  PubMed  Google Scholar 

  22. 22.

    Bertrand B, Honeyman CS, Emparanza A, McGurk M, Ousmane Hamady IE, Schmidt A, et al. Twenty-five years of experience with the submental flap in facial reconstruction. Plast Reconstr Surg. 2019;143(6):1747–58.

    CAS  Article  PubMed  Google Scholar 

  23. 23.

    Caldron PH, Impens A, Pavlova M, Groot W. A systematic review of social, economic and diplomatic aspects of short-term medical missions. BMC Health Serv Res. 2015;15(1):1–12.

    Article  Google Scholar 

  24. 24.

    Martiniuk AL, Manouchehrian M, Negin JA, Zwi AB. Brain Gains: a literature review of medical missions to low and middle-income countries. BMC Health Serv Res. 2012;12:134.

  25. 25.

    Sykes KJ. Short-term medical service trips: a systematic review of the evidence. Am J Public Health. 2014;104(7):e38–48 Available from:

    Article  Google Scholar 

  26. 26.

    Hendriks TCC, Botman M, Rahmee CNS, Ket JCF, Mullender MG, Gerretsen B, et al. Impact of short-term reconstructive surgical missions: a systematic review. BMJ Glob Health. 2019;4(2):e001176.

    Article  PubMed  PubMed Central  Google Scholar 

  27. 27.

    Banda CH, Georgios P, Narushima M, Ishiura R, Fujita M, Goran J. Challenges in global reconstructive microsurgery: the sub-Saharan african surgeons’ perspective. JPRAS Open. 2019;20:19–26.

    Article  PubMed  PubMed Central  Google Scholar 

  28. 28.

    Huijing MA, Marck KW, Combes J, Mizen KD, Fourie L, Demisse Y, et al. Facial reconstruction in the developing world: a complicated matter. Br J Oral Maxillofac Surg. 2011;49(4):292–6.

    Article  PubMed  Google Scholar 

  29. 29.

    Shamseer L, Moher D, Clarke M, Ghersi D, Liberati A, Petticrew M, et al. Preferred reporting items for systematic review and meta-analysis protocols (PRISMA-P) 2015: elaboration and explanation. BMJ. 2015;349(jan02 1):g7647.

    Article  Google Scholar 

  30. 30.

    Sideri S, Papageorgiou SN, Eliades T. Registration in the international prospective register of systematic reviews (PROSPERO) of systematic review protocols was associated with increased review quality. J Clin Epidemiol. 2018;100:103–10.

    Article  PubMed  Google Scholar 

  31. 31.

    Higgins JP, Thomas J, Chandler J, Cumpston M, Li T, Page MJ, Welch VA, editor(s). Cochrane Handbook for Systematic Reviews of Interventions Version 6.1 (updated September 2020). Cochrane. 2020. Available from Accessed 12 Dec 2020.

  32. 32.

    Moher D, Liberati A, Tetzlaff J, Altman DG. Preferred reporting items for systematic reviews and meta-analyses: the PRISMA statement. BMJ. 2009;339(jul21 1):b2535.

    Article  Google Scholar 

  33. 33.

    The World Bank. World Bank Country and Lending Groups [Internet]. [cited 2021 Jan 6]. Available from:

  34. 34.

    Richardson WS, Wilson MC, Nishikawa J, Hayward RS. The well-built clinical question: a key to evidence-based decisions. ACP J Club. 1995;123:A12–3.

    CAS  Article  Google Scholar 

  35. 35.

    Shanker MK, Rajan A, Hemant B, et al. Outcome of 1000 free flap head and neck reconstructions at a tertiary cancer care institute in India. Eur J Plast Surg. 2021;44:25–32.

  36. 36.

    Fischer JP, Nelson JA, Sieber B, Cleveland E, Kovach SJ, Wu LC, et al. Free tissue transfer in the obese patient. Plast Reconstr Surg. 2013;131(5):681e–92e.

    CAS  Article  Google Scholar 

  37. 37.

    Perisanidis C, Herberger B, Papadogeorgakis N, Seemann R, Eder-Czembirek C, Tamandl D, et al. Complications after free flap surgery: do we need a standardized classification of surgical complications? Br J Oral Maxillofac Surg. 2012;50(2):113–8.

    Article  PubMed  Google Scholar 

  38. 38.

    Sterne JAC, Savović J, Page MJ, Elbers RG, Blencowe NS, Boutron I, et al. RoB 2: a revised tool for assessing risk of bias in randomised trials. BMJ. 2019;28:l4898.

    Article  Google Scholar 

  39. 39.

    Sterne JA, Hernán MA, Reeves BC, Savović J, Berkman ND, Viswanathan M, et al. ROBINS-I: a tool for assessing risk of bias in non-randomised studies of interventions. BMJ. 2016;12:i4919.

    Article  Google Scholar 

  40. 40.

    National Heart Lung & Blood Institute. Managing blood cholesterol in adults systematic evidence review from the cholesterol expert panel, 2013. US Dept Heal Hum Serv [Internet]. 2013. Available from: Accessed 15 June 2021.

  41. 41.

    Howick J, Chalmers I, Glasziou P, Greenhalgh T, Heneghan C, Liberati A, Moschetti I, Phillips B, Thornton H. “Explanation of the 2011 Oxford Centre for Evidence-Based Medicine (OCEBM) Levels of Evidence (Background Document)”. Oxford Centre for Evidence-Based Medicine. Available from: Accessed 12 Dec 2020.

  42. 42.

    DerSimonian R, Laird N. Meta-analysis in clinical trials. Control Clin Trials. 1986;7(3):177–88.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  43. 43.

    Guyatt GH, Oxman AD, Vist GE, Kunz R, Falck-Ytter Y, Alonso-Coello P, et al. GRADE: an emerging consensus on rating quality of evidence and strength of recommendations. BMJ. 2008;336(7650):924–6.

    Article  PubMed  PubMed Central  Google Scholar 

  44. 44.

    Matošević M, Pegan A, Sambunjak D, Solter D, Vagić D, Rašić I, et al. Significance of anthropometric and nutritive factors in oral and oropharyngeal cancer patients undergoing free flap reconstruction. J Oral Maxillofac Surg. 2020;78(4):644–50.

    Article  PubMed  Google Scholar 

Download references


The authors would like to express our warmest gratitude to the staff and volunteers working for Project Harar in Ethiopia and the UK. We would also like to express our thanks to the plastic surgery team at Yekatit-12 Hospital and Medical College, Addis Ababa, for their ongoing support and engagement.


There are no sources of additional funding for this research.

Author information




All of the listed authors have made a substantial contribution towards the development of this protocol. CH conceived the study, reviewed the protocol design and registration, took part in the manuscript drafting and completion and gave specialist input relating to microsurgery and STSCs in LMICs. HdB developed the search strategy and data analysis section, reviewed the protocol design and registration, took part in the manuscript drafting and completion. DB, VP, MB, FA, GW, MM, DM, ME and VP provided expertise in the methodology and clinical area covered. HdB and UC developed the search strategy and data extraction policy. HdB established the statistical approach contained within this protocol. All authors also drafted and edited the manuscript and approved the final version prior to submission. All authors act as guarantors of this protocol.

Authors’ information

All of the authors have volunteered for the non-governmental organisation Project Harar, which conducts STSCs. VP and MM are trustees for Project Harar, and both sit on the board of directors.

Corresponding author

Correspondence to Henry T. de Berker.

Ethics declarations

Ethics approval and consent to participate

Not applicable

Consent for publication

Not applicable

Competing interests

All of the authors have volunteered for the non-governmental organisation, Project Harar, which conducts STSCs. VP and MM are trustees for Project Harar, and both sit on the board of directors.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Additional file 1.

PRISMA-P Checklist.

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

de Berker, H.T., Čebron, U., Bradley, D. et al. Protocol for a systematic review of outcomes from microsurgical free-tissue transfer performed on short-term collaborative surgical trips in low-income and middle-income countries. Syst Rev 10, 245 (2021).

Download citation


  • Free flap
  • Free tissue transfer
  • Microsurgery
  • Short-term collaborative surgical trips
  • Surgical missions
  • Low-income and middle-income countries
  • Resource limited settings