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The role of saline irrigation prior to wound closure in the reduction of surgical site infection: protocol for a systematic review and meta-analysis



Surgical site infection describes an infectious complication of surgical wounds. This single complication is thought to occur in close to 20% of surgical cases. This complication has been described in all kinds of surgical procedure including minimally invasive procedures. Wound irrigation is frequently used as a means of reducing surgical site infection. However, there is lack of solid evidence to support routine wound irrigation. The aim of this review is to provide evidence for the efficacy of routine wound irrigation with normal saline in preventing surgical site infection. The rate of surgical site infection in cases with and without wound irrigation will be analyzed.


Systematic literature searches will be conducted to identify all published and unpublished studies. The following databases will be searched for citations from inception to present: MEDLINE (via PubMed), Embase (via Embase), and CENTRAL (via the Cochrane library). The search strategy will be developed by the research team in collaboration with an experienced librarian and checked by a referee according to the Peer Review of Electronic Search Strategies (PRESS) guideline. A draft of the PubMed search strategy could be (irrigation[tiab] OR “Therapeutic Irrigation”[mesh] OR lavage[tiab]) AND (saline[tiab] OR “Sodium Chloride”[mesh] OR sodium chloride[tiab]) NOT (“Comment” [Publication Type] OR “Letter” [Publication Type] OR “Editorial” [Publication Type]). No time limits will be set. The reference lists of eligible articles will be hand searched. Relevant data will be extracted from eligible studies using a previously designed data extraction sheet. Relative risks will be calculated for binary outcomes and mean differences or standardized mean differences, if necessary, for continuous outcomes. For all measures, 95% confidence levels will be calculated. Both arms would be compared with regard to the rate of surgical site infection within 30 days following surgery. We will report the review using the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) statement.


This review aims at investigating the value of routine wound irrigation using normal saline in preventing surgical site infection.

Systematic review registration

PROSPERO: CRD42018082287

Peer Review reports



According to the World Health Organization (WHO), healthcare-associated infections (HAIs) acquired by patients when receiving care is the most frequent adverse event worldwide. Urinary tract infection, pneumonia, bloodstream, and wound infections with microorganisms represent the most common HAIs [1]. Surgical site infection (SSI) describes an infectious complication of surgical wounds. With an incidence of about 20%, SSI has been shown to be the most common HAI [2]. SSI surveillance report from the European Centre for Disease Prevention and Control for 2011 indicated a cumulative SSI incidence of 9.5% for colorectal surgery, 1.4% for cesarean section, and 1.0% for cholecystectomy [3].

SSI is a potential complication associated with any type of surgery irrespective of access (minimal invasive or open) or surgical discipline. Although SSI is thought to be preventable, it still represents a major cause of morbidity and substantial economic burden on the health system [4]. Many study groups and international guidelines have suggested measures to prevent or at least reduce the rate of SSI [1, 5,6,7,8]. The use of prophylactic single-shot antibiotics at the beginning of surgery prior to skin incision, minimally invasive access with less tissue trauma, and the use of wound protectors have been described as means of reducing SSI [5, 9,10,11].

Wound irrigation (WI) describes the flow of a solution across the surface of a surgical incision prior to wound closure [12]. Wound irrigation is intended to cleanse the wound physically by removing cellular debris and trapped fluids. Wound irrigation might reduce the level of bacterial contamination by flushing off bacteria from the wound surface [13].

Different forms of WI have been described in the medical literature. WI can be achieved using normal saline, antibiotic solutions, and solutions with antiseptic agents [14,15,16]. Despite the large number of publications on WI, no single method or solution has been shown to be superior. Besides, there seems to be conflicting data on the role of WI on SSI [17, 18]. In fact, some guidelines do not recommend routine WI [5, 7]. This trend can be observed across different surgical disciplines [19, 20].

In our department of surgery at a university hospital, WI using normal saline is routinely performed and the rate of SSI is very low. Current data on the role of WI as a means of reducing or preventing SSI is conflicting. Based on our clinical experience, we hypothesize that WI with normal saline might reduce the rate of SSI. However, no systematic review to the best of our knowledge has so far investigated WI with normal saline alone. We therefore intend to investigate the potential role of WI with normal saline in preventing SSI by performing a systematic review of randomized controlled trials (RCTs).


We aim to investigate the efficacy of WI with normal saline in reducing surgical site infection. The rate of SSI in patients following wound irrigation with normal saline will be compared to that of patients without wound irrigation prior to wound closure.


This protocol adheres to the Preferred Reporting Items for Systematic Review and Meta-Analysis-Protocols (PRISMA-P) [21]. Any amendments to this protocol will be reflected in an update to the PROSPERO registration.

Eligibility criteria

RCTs investigating the rate of SSI in using normal saline vs. no irrigation prior to wound closure following abdominal surgery will be included. Only studies published in English and German language will be included. Relevant studies in languages other than English will be considered if an English translation is available. In such cases, a translation will be requested from the corresponding authors. All available publications irrespective of date of publication will be considered.


All patients undergoing abdominal surgery irrespective of diagnosis, procedure, and discipline will be considered for inclusion. Patients undergoing minimally invasive surgery will be included if a mini-laparotomy of more than 3 cm was performed, e.g., for specimen retrieval. Patients undergoing natural orifice procedures (NOTES) will not be included. Patients undergoing both elective and emergency surgery will be included. No differences will be made between patients with or without drains. The same role holds for the use of wound protectors.


The study group will include all patients who underwent saline irrigation of the surgical incision prior to wound closure. All forms of saline wound irrigation with or without the use of a pressure device or syringe will be included in this intervention arm. The control group will include patients without irrigation.


All comparisons will be considered.


Primary outcome will be SSI. As secondary outcomes, we will consider length of stay (LOS), rate of re-intervention, rate of re-admission, overall morbidity, mortality, quality of life, and the resource use for treatment.

Study designs

We will only include (quasi-) RCTs in our systematic review. Systematic reviews related to the topic will be retained to investigate their references for further eligible studies.

Information sources

We will conduct a systematic literature search to identify all published and unpublished studies. The following databases will be searched for citations from inception to present: MEDLINE (via PubMed), Embase (via Embase), and CENTRAL (via the Cochrane library). We will search manually for additional studies by cross-checking the reference lists of all included primary studies and lists of relevant systematic reviews.

We will not apply any limitations regarding language, publication status, and publication date when searching for eligible studies.

Search strategy

The search strategy will be developed by the research team in collaboration with an experienced librarian and checked by a referee according to the Peer Review of Electronic Search Strategies (PRESS) guideline. A draft of the PubMed search strategy can be found below:

(irrigation[tiab] OR “Therapeutic Irrigation”[mesh] OR lavage[tiab]) AND (saline[tiab] OR “Sodium Chloride”[mesh] OR sodium chloride[tiab])


((randomized controlled trial[pt] OR controlled clinical trial[pt] OR randomized[tiab] OR randomised[tiab] OR placebo[tiab] OR clinical trials as topic[mesh:noexp] OR randomly[tiab] OR trial[ti] NOT (animals[mh] NOT humans [mh]))) NOT (“Comment” [Publication Type] OR “Letter” [Publication Type] OR “Editorial” [Publication Type])

Data management

The search results will be uploaded and managed using Microsoft Excel.

Selection process

The title and abstract of each article will be screened and assessed against predetermined inclusion criteria by two independent investigators. Full texts of all potentially relevant articles and those without an available abstract will be assessed for inclusion by two reviewers independently. Discrepancies will be resolved by consensus or consulting a third investigator. The corresponding authors of eligible articles will be contacted for clarification where necessary. We will record the reasons for exclusion and report the study selection process using the PRISMA flow diagram [22]. A list of excluded studies will be provided.

Data collection process

A data extraction sheet will be designed and tested. Two reviewers will independently extract data from the included studies. Any disagreements will be resolved via discussion or by involving a third reviewer for arbitration.

Data items

We will collect data on patient’s demographics (age, sex, body mass index), relevant medical conditions (ASA score, diabetes, immune status), type of surgical procedure and means of access (laparoscopic or open), information on lifestyle, e.g., smoking, and perioperative data including procedure-associated information like type of surgery, duration of surgery, use of single-shot antibiotics, and use of wound drains. Furthermore, we will extract the number of randomized patients included in the analysis.

The primary outcome will be the number of SSI. Secondary outcomes will include mean or median LOS, number of re-intervention, specific medical treatment for SSI (e.g., antibiotics), number of readmission, overall morbidity, number of deaths, and resource use (e.g., hospital length of stay, costs). In case outcome data are missing, we will contact study authors and request the data.

Risk of bias in individual studies

We will use the revised Cochrane Risk of Bias tool to evaluate all included studies for risk of bias [23]. Items will be rated as low, high, or unclear risk of bias. Independently, two reviewers will assess the risk of bias of all included studies. We will assess the risk of bias on outcome level. For each assessment, we will provide a support for judgment. Any disagreements will be resolved through discussion. If both reviewers cannot find a consensus, we will involve a third reviewer.

Summary measures and synthesis

Relative risks will be calculated for binary outcomes and mean differences or standardized mean differences, if necessary, for continuous outcomes. For all measures, 95% confidence levels will be calculated.

Clinical and statistical heterogeneity between studies will be assessed by two reviewers. For the assessment of statistical heterogeneity, I2 will be calculated. In the absence of clinical heterogeneity, and in the presence of statistical heterogeneity (I2 > 50%), we will use a random-effects model. In case of no clinical or statistical heterogeneity, we will apply a fixed-effect model. We will obtain pooled estimates of treatment effect using RevMan 5 software.

We do not plan any additional analyses, such as subgroup or sensitivity analyses. However, we may decide to perform post hoc analyses given appropriate numbers.


If there are 10 or more studies included in the meta-analysis, we will investigate publication bias using funnel plots and Egger’s test [24].

Confidence in cumulative evidence

Summary of finding tables will be prepared for summarizing confidence across studies for all patient relevant outcomes. For grading the quality of evidence, the five GRADE domains, risk of bias, indirectness, inconsistency, imprecision, and publication bias, will be judged. The quality of the body of evidence will be assessed by two reviewers independently using the GRADEpro GDT software.


Two out of ten patients undergoing abdominal surgery will suffer from SSI, which causes an increase in overall morbidity rate [2]. Besides, SSI might lead to prolongation of LOS [25, 26]. The economic burden imposed on the health system by SSI cannot be overemphasized. Although many institutions implemented measures to reduce the risk of SSI, this single complication is still a cause of serious morbidity and expenses [27].

Although wound irrigation has been widely investigated in association with surgical site infection, consistent data supporting the benefit of WI is lacking. One reason for this trend might be the heterogeneity of the study population with regard to wound irrigation. Antibiotic solution, solution with antiseptic agents, normal saline, and other combinations have so far been used for wound irrigation. This observation was confirmed in a recently published systematic review by Mueller et al. [28].

Most solutions used for irrigation except saline are not inert. It is thus possible that substances in the irrigation solution might negatively affect wound healing thereby predisposing to SSI. We routinely perform wound irrigation using saline in our department of abdominal surgery. Our clinical experience suggests a potential reduction in the rate of SSI following routine WI with saline. Currently, there is limited evidence on the role of normal saline irrigation on the rate of SSI.

This systematic review will compute data from RCTs to clarify the role of routine WI with normal saline in the reduction or prevention of SSI. The risk of SSI in patients managed with normal saline irrigation will be compared to that of controls without WI. Secondary endpoints like the rate of morbidity, length of stay, and overall resource use of treatment will be investigated.

The results generated in the planned systematic review might provide solid evidence to rethink our routine clinical processes or adjust the current recommendations for the prevention of surgical site infection. It is also conceivable that the planned systematic review might not indicate any trend, which would reveal the need for further investigation, e.g., via additional RCTs.


  1. 1.

    Organization WH: Global guidelines for the prevention of surgical site infection: World Health Organization; 2016.

    Google Scholar 

  2. 2.

    Allegranzi B, Bagheri Nejad S, Combescure C, Graafmans W, Attar H, Donaldson L, Pittet D. Burden of endemic health-care-associated infection in developing countries: systematic review and meta-analysis. Lancet. 2011;377(9761):228–41.

    Article  Google Scholar 

  3. 3.

    Tommi Kärki, Carl Suetens. SossiiESECfDPaCaO. Accessed on Oct 8 2017.

  4. 4.

    Leaper DJ, van Goor H, Reilly J, Petrosillo N, Geiss HK, Torres AJ, Berger A. Surgical site infection - a European perspective of incidence and economic burden. Int Wound J. 2004;1(4):247–73.

    Article  Google Scholar 

  5. 5.

    Anderson DJ, Podgorny K, Berrios-Torres SI, Bratzler DW, Dellinger EP, Greene L, Nyquist AC, Saiman L, Yokoe DS, Maragakis LL, et al. Strategies to prevent surgical site infections in acute care hospitals: 2014 update. Infect Control Hosp Epidemiol. 2014;35(6):605–27.

    Article  Google Scholar 

  6. 6.

    Gilmore O, Martin T. Aetiology and prevention of wound infection in appendicectomy. Br J Surg. 1974;61(4):281–7.

    CAS  Article  Google Scholar 

  7. 7.

    Leaper D, Burman-Roy S, Palanca A, Cullen K, Worster D, Gautam-Aitken E, Whittle M, Guideline Development G: Prevention and treatment of surgical site infection: summary of NICE guidance. BMJ 2008, 337:a1924.

    Article  Google Scholar 

  8. 8.

    Saves GfssSS, Lives. Geneva: World Health Organization; 2009 Accessed 8 Oct 2017.

  9. 9.

    Classen DC, Evans RS, Pestotnik SL, Horn SD, Menlove RL, Burke JP. The timing of prophylactic administration of antibiotics and the risk of surgical-wound infection. N Engl J Med. 1992;326(5):281–6.

    CAS  Article  Google Scholar 

  10. 10.

    Bratzler DW, Dellinger EP, Olsen KM, Perl TM, Auwaerter PG, Bolon MK, Fish DN, Napolitano LM, Sawyer RG, Slain D, et al. Clinical practice guidelines for antimicrobial prophylaxis in surgery. Surg Infect. 2013;14(1):73–156.

    Article  Google Scholar 

  11. 11.

    Gheorghe A, Roberts TE, Pinkney TD, Bartlett DC, Morton D, Calvert M, West Midlands Research C, Investigators RT: The cost-effectiveness of wound-edge protection devices compared to standard care in reducing surgical site infection after laparotomy: an economic evaluation alongside the ROSSINI trial. PLoS One 2014, 9(4):e95595.

    Article  Google Scholar 

  12. 12.

    Whiteside OJ, Tytherleigh MG, Thrush S, Farouk R, Galland RB. Intra-operative peritoneal lavage--who does it and why? Ann R Coll Surg Engl. 2005;87(4):255–8.

    CAS  Article  Google Scholar 

  13. 13.

    Mangram AJ, Horan TC, Pearson ML, Silver LC, Jarvis WR. Guideline for prevention of surgical site infection, 1999. Centers for disease control and prevention (CDC) hospital infection control practices advisory committee. Am J Infect Control. 1999;27(2):97–132 quiz 133-134; discussion 196.

    CAS  Article  Google Scholar 

  14. 14.

    Andersen B, Korner B, Ostergaard AH. Topical ampicillin against wound infection after colorectal surgery. Ann Surg. 1972;176(2):129.

    CAS  Article  Google Scholar 

  15. 15.

    Andersen JR, Burcharth F, Larsen HW, Røder O, Andersen B. Polyglycolic acid, silk, and topical ampicillin: their use in hernia repair and cholecystectomy. Arch Surg. 1980;115(3):293–5.

    CAS  Article  Google Scholar 

  16. 16.

    De Jong T, Vierhout R, Van Vroonhoven T. Povidone-iodine irrigation of the subcutaneous tissue to prevent surgical wound infections. Surg Gynecol Obstet. 1982;155(2):221–4.

    CAS  PubMed  Google Scholar 

  17. 17.

    Galle PC, Homesley HD. Ineffectiveness of povidone-iodine irrigation of abdominal incisions. Obstet Gynecol. 1980;55(6):744–6.

    CAS  PubMed  Google Scholar 

  18. 18.

    Gill RS, Al-Adra DP, Campbell S, Olson DW, Rowe BH. Povidone-iodine irrigation of subcutaneous tissues may decrease surgical site infections in elective colorectal operations: a systematic review. Gastroenterology Res. 2011;4(3):97.

    CAS  PubMed  PubMed Central  Google Scholar 

  19. 19.

    Stevenson TR, Thacker JG, Rodeheaver GT, Bacchetta C, Edgerton MT, Edlich RF. Cleansing the traumatic wound by high pressure syringe irrigation. J Am Coll Emerg Physicians. 1976;5(1):17–21.

    CAS  Article  Google Scholar 

  20. 20.

    Lord JW, LaRaja RD, Daliana M, Gordon MT. Prophylactic antibiotic wound irrigation in gastric, biliary, and colonic surgery. Am J Surg. 1983;145(2):209–12.

    Article  Google Scholar 

  21. 21.

    Moher D, Liberati A, Tetzlaff J, Altman DG, Group P. Preferred reporting items for systematic reviews and meta-analyses: the PRISMA statement. PLoS Med. 2009;6(7):e1000097.

    Article  Google Scholar 

  22. 22.

    Liberati A, Altman DG, Tetzlaff J, Mulrow C, Gotzsche PC, Ioannidis JP, Clarke M, Devereaux PJ, Kleijnen J, Moher D. The PRISMA statement for reporting systematic reviews and meta-analyses of studies that evaluate health care interventions: explanation and elaboration. PLoS Med. 2009;6(7):e1000100.

    Article  Google Scholar 

  23. 23.

    Higgins JP, Altman DG, Gotzsche PC, Juni P, Moher D, Oxman AD, Savovic J, Schulz KF, Weeks L, Sterne JA, et al. The Cochrane Collaboration’s tool for assessing risk of bias in randomised trials. BMJ. 2011;343:d5928.

    Article  Google Scholar 

  24. 24.

    Egger M, Davey Smith G, Schneider M, Minder C. Bias in meta-analysis detected by a simple, graphical test. BMJ. 1997;315(7109):629–34.

    CAS  Article  Google Scholar 

  25. 25.

    Kirkland KB, Briggs JP, Trivette SL, Wilkinson WE, Sexton DJ. The impact of surgical-site infections in the 1990s: attributable mortality, excess length of hospitalization, and extra costs. Infect Control Hosp Epidemiol. 1999;20(11):725–30.

    CAS  Article  Google Scholar 

  26. 26.

    Merle V, Germain JM, Chamouni P, Daubert H, Froment L, Michot F, Teniere P, Czernichow P. Assessment of prolonged hospital stay attributable to surgical site infections using appropriateness evaluation protocol. Am J Infect Control. 2000;28(2):109–15.

    CAS  Article  Google Scholar 

  27. 27.

    de Lissovoy G, Fraeman K, Hutchins V, Murphy D, Song D, Vaughn BB. Surgical site infection: incidence and impact on hospital utilization and treatment costs. Am J Infect Control. 2009;37(5):387–97.

    Article  Google Scholar 

  28. 28.

    Mueller TC, Loos M, Haller B, Mihaljevic AL, Nitsche U, Wilhelm D, Friess H, Kleeff J, Bader FG. Intra-operative wound irrigation to reduce surgical site infections after abdominal surgery: a systematic review and meta-analysis. Langenbecks Arch Surg. 2015;400(2):167–81.

    Article  Google Scholar 

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Author information




PCA was in charge of the conception. DP and PCA designed the protocol and wrote the first draft. TR, JD, JDR, KZ, and HZ contributed to the critical revision of the manuscript for methodological and intellectual content. All authors approved the final version of the submitted manuscript.

Corresponding author

Correspondence to Peter C. Ambe.

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Pieper, D., Rombey, T., Doerner, J. et al. The role of saline irrigation prior to wound closure in the reduction of surgical site infection: protocol for a systematic review and meta-analysis. Syst Rev 7, 152 (2018).

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  • Surgical Site Infection
  • Wound Irrigation (WI)
  • Peer Review Of Electronic Search Strategies (PRESS)
  • Publication Type
  • Single Complication