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Colorectal cancer among persons with HIV: protocol for a systematic review and meta-analysis
Systematic Reviews volume 4, Article number: 72 (2015)
As persons with HIV live longer, data regarding the epidemiology of colorectal cancer are required to optimize the long-term management of these patients. The purpose of this systematic review and meta-analysis is to synthesize evidence regarding the incidence of colorectal cancer in persons with HIV.
Our primary outcome is the standardized incidence ratio of colorectal cancer among persons with HIV relative to rates in persons not living with HIV. Our secondary objectives are to summarize the evidence for differences with respect to stage at diagnosis, site of disease, and mortality due to colorectal cancer. We will search electronic bibliographic databases from their inception date, as well as conference proceedings and reference lists of included articles. Two investigators will independently screen citations and full-text articles, conduct data abstraction, and appraise study quality. We will examine clinical, methodological, and statistical heterogeneity among studies prior to conducting meta-analysis. Random effects meta-analysis methods will be employed to estimate standardized incidence ratios. These data will inform the development of guidelines for colorectal cancer screening in persons with HIV.
Systematic review registration
Rates of AIDS-defining cancers such as Kaposi’s sarcoma and non-Hodgkin’s lymphoma have declined markedly among persons with HIV infection in the years following the introduction of combination antiretroviral therapy (cART) [1,2]. However, the incidence of certain cancers not traditionally associated with HIV has increased more than threefold in this population over the same period, such that these non-AIDS-defining cancers now account for an increasing proportion of deaths among persons with HIV [2-5]. Consequently, research regarding the incidence, natural history, and outcomes of cancers not historically associated with HIV infection is required to inform patient care and develop age-appropriate screening guidelines in this population.
Colorectal cancer (CRC) is the third leading cause of cancer-related death in North America, with an estimated 9,200 Canadians dying from this disease in 2012 [6,7]. As individuals with HIV are living longer, it is expected that the number of cases of CRC among this population will increase simply by virtue of the age-related risk for this disease. Yet, in contrast to human papillomavirus-associated rectal cancer, very little is known about the epidemiology of CRC in persons with HIV, and the available literature is inconsistent with respect to the risk of this disease in persons with HIV. Specifically, the standardized incidence ratio for CRC in persons with HIV compared with the general population has ranged from 0.45 to 2.3 in various studies [1,8-16]. Similarly, although small studies have found that CRC is diagnosed at a younger age relative to non-HIV-infected individuals, these findings were subsequently disputed by a larger study that found similar standardized incidence ratios for CRC when stratification by age was undertaken . In addition, the prevalence of adenomatous polyps in persons with HIV exceeds that of the general population in most, but not all, studies [17-21].
Previously published meta-analyses of studies reported up to March 2007 and March 2009 found standardized incidence ratios and 95% confidence intervals of CRC in persons with HIV of 0.97 (0.78 to 1.19)  and 1.1 (0.69 to 1.7) , respectively. However, new studies have been published since the last review was undertaken [3,5,24-27], and a systematic review of the clinical features of CRC in persons with HIV relative to uninfected individuals has not been reported. Accordingly, we plan to conduct a systematic review and meta-analysis of publications reporting the risk of CRC in persons with HIV, using estimates of the standardized incidence ratio relative to rates in a referent population of persons not living with HIV. Our secondary objectives are to summarize the evidence for differences between persons with and without HIV with respect to stage at diagnosis, site of disease occurrence, and treatment outcomes, focusing specifically on mortality due to CRC. Such data are required to inform screening guidelines for persons with HIV and optimize the health of these patients.
Design and scope
We will conduct a systematic review and meta-analysis of the epidemiology and clinical presentation of CRC in persons with HIV.
Our primary outcome is the standardized incidence ratio of CRC among persons with HIV relative to rates in persons not infected with HIV. As secondary outcomes, we will examine stage of diagnosis, the standardized mortality ratio as well, site of disease, and treatment outcomes among persons with HIV relative to a referent HIV-uninfected population.
We will include any published peer-reviewed and gray literature (e.g., conference abstracts, white papers) that provide a comparison of the incidence of CRC in adults with HIV and a referent population of persons who are not living with HIV. Included studies will report information sufficient to obtain standardized incidence ratios and 95% confidence intervals, including the total number of adults with HIV observed, the duration of follow-up, the observed number of CRCs occurring during follow-up in adults with HIV (identified using disease registries), and corresponding data for the HIV-uninfected comparator population. Prospective and retrospective cohort studies will be eligible for inclusion. We will include cross-sectional, case–control, and cohort studies that provide data regarding risk factors for CRC in persons with HIV, stage at diagnosis, site of disease, treatment mode, and CRC mortality. When two or more publications from one institution or cohort are available, we will review each article to consider the extent of duplication in the reported outcomes. For our main outcome, we will include results from the most recent publication only. Publications with overlapping populations will be retained if they provide information on outcomes that complements or differs from the most recent report. We will make no restrictions according to country or language of publication. We will exclude animal studies, case reports, case series, and randomized trials of CRC treatment, as well as studies where CRC is identified by self-report or patient questionnaire. In addition, we will exclude studies comparing the incidence, natural history, and treatment outcome of human papillomavirus-associated anal and rectal cancers.
We will develop a search strategy in consultation with an information scientist. We will search the following electronic bibliographic databases from inception onwards: Ovid MEDLINE, Ovid EMBASE, Cumulative Index to Nursing and Allied Health Literature (CINAHL), Scopus, and Web of Science (see Additional file 1 for MEDLINE search strategy). The search will include the terms HIV, colorectal cancer, AIDS, and incidence. We will also search abstracts for the last 5 years from the following HIV/AIDS conferences: Conference on Retroviruses and Opportunistic Infections, International AIDS Conference, and International AIDS Society (IAS). Finally, we will scan the bibliographies of all studies meeting the inclusion criteria for additional relevant citations.
We will import all citations obtained using the search strategy into DistillerSR™ to facilitate study screening and selection, de-duplicating citations prior to undertaking the abstract review. Study selection will proceed according to the three stages as described below:
Stage 1: Broad overview
During this stage, two reviewers will independently scan all titles and abstracts of all citations for articles that obviously do not merit further consideration. Specific points that will be considered when reviewing the title and abstract include whether the study includes adults with HIV, examines the incidence of CRC relative to a referent HIV-uninfected population, and/or provides the observed and expected number of cases of CRC in persons with HIV and a referent population.
Stage 2: Pilot testing of customized checklist for study selection
A customized form reflecting the previously described inclusion will be pilot tested by two reviewers. Specifically, the data collection form will be developed and stored on DistillerSR™ software and will be applied by two reviewers independently to a sample of 50 abstracts to ensure consistency of use and clarity of the instrument. A Cohen’s kappa statistic  will be estimated to measure inter-rater reliability, and screening will begin when >60% agreement is achieved.
Stage 3: Assessment of remaining studies for inclusion
Abstracts of studies remaining following stage 1 will be reviewed independently by two reviewers using the customized checklist developed in stage 2. We will ascertain the relevance of the studies without blinding to author names, institutions, or journal of publication. Differences in opinion will be resolved by consensus and discussion with a third author in situations where consensus cannot be reached. In cases where abstracts are not provided, are unclear, or there is any other reason for uncertainty, the full article will be obtained before making the decision regarding eligibility for inclusion. When abstracts are not available in English, the assistance of translators will be obtained. Full-text versions of the papers identified from the abstract screening process will be subsequently retrieved and assessed independently by two reviewers for inclusion in the review.
The same investigators involved in study selection will independently extract data from eligible studies using a pre-designed data extraction form. The data extraction form will be pilot tested with a sample of five to ten studies to ensure clarity and consistency. We will abstract data regarding study methodology and sociodemographic characteristics (Table 1) to facilitate exploration of potential sources of heterogeneity with stratified analyses and/or meta-regression. We will also extract available data comparing the clinical characteristics of CRC among persons with and without HIV, including age at diagnosis, stage of disease (I, II, III, IV), disease grade (well differentiated, moderately differentiated, poorly differentiated, undifferentiated), site of disease (right, left, rectosigmoid, synchronous), nature of treatment received (surgery, radiofrequency ablation, cryosurgery, chemotherapy, radiation, targeted therapy), and outcomes (remission, local recurrence, regional recurrence, distant recurrence, unknown site recurrence, and death). Discrepancies arising during the data abstraction process will be resolved by consensus or discussion with a third party if agreement cannot be reached.
The same reviewers involved in data abstraction will independently evaluate the quality of eligible studies using the Newcastle-Ottawa Scale [29,30]. We decided a priori that studies meeting seven or more of the nine Newcastle-Ottawa Scale criteria were to be considered high quality. We will also use a modified version of the framework developed by Hayden and colleagues [31,32] to evaluate study participation, study attrition, and outcome measurement.
Assessment of heterogeneity
We will examine clinical, methodological, and statistical heterogeneity among studies prior to conducting meta-analysis. Clinical heterogeneity will be evaluated by reviewing studies for differences in patient characteristics that may influence the risk of CRC, such as age and sex, as well as whether the study was conducted in the pre- or post-cART era. We will assess studies for methodological heterogeneity by examining differences in HIV status ascertainment and outcomes assessment, particularly for the secondary outcomes. Another source of methodological heterogeneity that will be considered is whether standardized incidence ratios are crude or adjusted estimates, methods (if any) of confounder adjustment, and the confounders that have been adjusted for in each study. Finally, we will quantify the proportion of cross-study variability that is due to heterogeneity rather than chance using the I2 statistic .
We will use random effects meta-analysis to estimate a pooled effect size of the standardized incidence ratio for CRC. In the event of extensive heterogeneity (I2 > 60%), we will conduct subgroup analyses or meta-regression if there are more than ten studies providing data regarding the primary outcome. Specific variables we will examine in subgroup analyses or meta-regression include age, study quality, sex, era of rate estimation (pre- or post-cART era), and mean CD4+ count. If applicable, we will also compare studies reporting crude versus adjusted standardized incidence ratios and/or studies with varying types and degrees of confounder adjustment. We will use influence analyses to ascertain whether individual studies were particularly influential on summary estimates of standardized incidence ratios. Secondary outcomes will be summarized descriptively and not meta-analyzed, with the exception of standardized mortality ratios if there are sufficient data.
If at least ten studies are available, we will test for reporting and other bias using funnel plots with Egger’s test . However, because funnel plot asymmetry may not be due to reporting bias, we will attempt to discern the possible reasons for asymmetry, such as poor methodological quality, differences in the extent of confounding control between smaller and larger studies, and true heterogeneity in the included studies. All statistical analyses will be conducted using Comprehensive Meta-Analysis  and STATA version 13 (Stata Corporation, College Park, TX, USA).
Our systematic review will provide important information regarding the epidemiology of CRC in persons with HIV relative to non-HIV-infected persons. The results of this review will be of relevance to clinicians, public health practitioners, and individuals living with HIV. Our knowledge dissemination strategies will therefore include presentations to the community of persons with HIV, presentations at HIV conferences, and publication in a peer-reviewed journal. In addition, the results of this systematic review will be presented to stakeholders participating in a future Delphi panel examining whether existing guidelines for CRC screening are adequate for persons with HIV.
Human immunodeficiency virus
Acquired immunodeficiency syndrome
Clifford GM, Polesel J, Rickenbach M, Dal Maso L, Keiser O, Kofler A, et al. Cancer risk in the Swiss HIV Cohort Study: associations with immunodeficiency, smoking, and highly active antiretroviral therapy. J Natl Cancer Inst. 2005;97:425–32.
Crum-Cianflone N, Hullsiek KH, Marconi V, Weintrob A, Ganesan A, Barthel RV, et al. Trends in the incidence of cancers among HIV-infected persons and the impact of antiretroviral therapy: a 20-year cohort study. AIDS. 2009;23:41–50.
Shiels MS, Pfeiffer RM, Gail MH, Hall HI, Li J, Chaturvedi AK, et al. Cancer burden in the HIV-infected population in the United States. J Natl Cancer Inst. 2011;103:753–62.
Bedimo R, Chen RY, Accortt NA, Raper JL, Linn C, Allison JJ, et al. Trends in AIDS-defining and non-AIDS-defining malignancies among HIV-infected patients: 1989–2002. Clin Infect Dis. 2004;39:1380–4.
Powles T, Robinson D, Stebbing J, Shamash J, Nelson M, Gazzard B, et al. Highly active antiretroviral therapy and the incidence of non-AIDS-defining cancers in people with HIV infection. J Clin Oncol. 2009;27:884–90.
Siegel R, Ma J, Zou Z, Jemal A. Cancer statistics, 2014. CA Cancer J Clin. 2014;64:9–29.
Canadian Cancer Society’s Steering Committee on Cancer Statistics. Canadian Cancer Statistics 2012. Toronto, ON: Canadian Cancer Society; 2012.
Engels EA, Pfeiffer RM, Goedert JJ, Virgo P, McNeel TS, Scoppa SM, et al. Trends in cancer risk among people with AIDS in the United States 1980–2002. AIDS. 2006;20:1645–54.
Grulich AE, Li Y, McDonald A, Correll PK, Law MG, Kaldor JM. Rates of non-AIDS-defining cancers in people with HIV infection before and after AIDS diagnosis. AIDS. 2002;16:1155–61.
Frisch M, Biggar RJ, Engels EA, Goedert JJ, AIDS-Cancer Match Registry Study Group. Association of cancer with AIDS-related immunosuppression in adults. JAMA. 2001;285:1736–45.
Dal Maso L, Franceschi S, Polesel J, Braga C, Piselli P, Crocetti E, et al. Risk of cancer in persons with AIDS in Italy, 1985–1998. Br J Cancer. 2003;89:94–100.
Herida M, Mary-Krause M, Kaphan R, Cadranel J, Poizot-Martin I, Rabaud C, et al. Incidence of non-AIDS-defining cancers before and during the highly active antiretroviral therapy era in a cohort of human immunodeficiency virus-infected patients. J Clin Oncol. 2003;21:3447–53.
Engels EA, Biggar RJ, Hall HI, Cross H, Crutchfield A, Finch JL, et al. Cancer risk in people infected with human immunodeficiency virus in the United States. Int J Cancer. 2008;123:187–94.
Patel P, Hanson DL, Sullivan PS, Novak RM, Moorman AC, Tong TC, et al. Incidence of types of cancer among HIV-infected persons compared with the general population in the United States, 1992–2003. Ann Intern Med. 2008;148:728–36.
Long JL, Engels EA, Moore RD, Gebo KA. Incidence and outcomes of malignancy in the HAART era in an urban cohort of HIV-infected individuals. AIDS. 2008;22:489–96.
Newnham A, Harris J, Evans HS, Evans BG, Møller H. The risk of cancer in HIV-infected people in southeast England: a cohort study. Br J Cancer. 2005;92:194–200.
Bini EJ, Green B, Poles MA. Screening colonoscopy for the detection of neoplastic lesions in asymptomatic HIV-infected subjects. Gut. 2009;58:1129–34.
Bini EJ, Park J, Francois F. Use of flexible sigmoidoscopy to screen for colorectal cancer in HIV-infected patients 50 years of age and older. Arch Intern Med. 2006;166:1626–31.
Kasapovic A, Boesecke C, Schwarze-Zander C, Anadol E, Vogel M, Hippe V, et al. Screening colonoscopy in HIV-infected patients: high rates of mucosal abnormalities in a German HIV-infected cohort. HIV Med. 2014;15:175–81.
Nayudu SK, Balar B. Colorectal cancer screening in human immunodeficiency virus population: are they at average risk? World J Gastrointest Oncol. 2012;4:259–64.
Hamada Y, Nagata N, Nishijima T, Shimbo T, Asayama N, Kishida Y, et al. Impact of HIV infection on colorectal tumors: a prospective colonoscopic study of Asian patients. J Acquir Immune Defic Syndr. 2014;65:312–17.
Grulich AE, van Leeuwen MT, Falster MO, Vajdic CM. Incidence of cancers in people with HIV/AIDS compared with immunosuppressed transplant recipients: a meta-analysis. Lancet. 2007;370:59–67.
Shiels MS, Cole SR, Kirk GD, Poole C. A meta-analysis of the incidence of non-AIDS cancers in HIV-infected individuals. J Acquir Immune Defic Syndr. 2009;52:611–22.
Guest JL, Rentsch CT, Rimland D. Comparison of colorectal cancer screening and diagnoses in HIV-positive and HIV-negative veterans. AIDS Care. 2014;26:1490–3.
Silverberg MJ, Chao C, Leyden WA, Xu L, Tang B, Horberg MA, et al. HIV infection and the risk of cancers with and without a known infectious cause. AIDS. 2009;23:2337–45.
Bedimo RJ, McGinnis KA, Dunlap M, Rodriguez-Barradas MC, Justice AC. Incidence of non-AIDS-defining malignancies in HIV-infected versus noninfected patients in the HAART era: impact of immunosuppression. J Acquir Immune Defic Syndr. 2009;52:203–8.
Franceschi S, Lise M, Clifford GM, Rickenbach M, Levi F, Maspoli M, et al. Changing patterns of cancer incidence in the early- and late-HAART periods: the Swiss HIV Cohort Study. Br J Cancer. 2010;103:416–22.
Viera AJ, Garrett JM. Understanding interobserver agreement: the kappa statistic. Fam Med. 2005;37:360–3.
Higgins JPT, Green S: Cochrane handbook for systematic reviews of interventions version 5.1.0. 2014, http://handbook.cochrane.org/. Accessed December 24, 2014.
Wells G, Shea B, O’Connell D, Peterson J, Welch V, Losos M, Tugwell P: The Newcastle-Ottawa Scale (NOS) for assessing the quality of nonrandomised studies in meta-analyses. 2013, http://www.ohri.ca/programs/clinical_epidemiology/oxford.asp. Accessed December 24, 2014.
Hayden JA, Côté P, Bombardier C. Evaluation of the quality of prognosis studies in systematic reviews. Ann Intern Med. 2006;144:427–37.
Hayden JA, van der Windt DA, Cartwright JL, Côté P, Bombardier C. Assessing bias in studies of prognostic factors. Ann Intern Med. 2013;158:280–6.
Borenstein M, Hedges LV, Higgins JPT, Rothstein HR. Introduction to meta-analysis. John Wiley & Sons, Ltd: West Sussex; 2009.
Egger M, Davey Smith G, Schneider M, Minder C. Bias in meta-analysis detected by a simple, graphical test. BMJ. 1997;315:629–34.
Borenstein M, Hedges LV, Higgins JPT, Rothstein HR: Comprehensive Meta-Analysis, Version 2.0 (2014). Engelwood, NJ: Biostat. Available from: http://www.meta-analysis.com/index.php
This project is supported by funds from the Ontario HIV Treatment Network. Ann N. Burchell and Tony Antoniou are supported by new investigator awards from the Canadian Institutes of Health Research.
The authors declare that they have no competing interests.
TA conceived the study. JND, TO, NK, AMT, AA, ANB, and TA were involved in designing the study. JND and TA wrote the draft protocol. TO, NK, AMT, AA, and ANB revised the manuscript critically for intellectual content. All authors read and approved the final manuscript and agree to be accountable for all aspects of the work.
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Nguemo, J.D., O’Neill, T.J., Kou, N. et al. Colorectal cancer among persons with HIV: protocol for a systematic review and meta-analysis. Syst Rev 4, 72 (2015). https://doi.org/10.1186/s13643-015-0054-y
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