The impact of different preanalytical methods related to CA 15-3 determination in frozen human blood samples: a systematic review

Table 1 Summary of the findings of the 18 eligible articles on CA 15-3 determination from thawed human biosamples

	No. of studies [reference]
Study type
Retrospective study	17 [22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38]
Prospective study	1 [39]
Biospecimen type
Serum	14 [22,23,24,25,26,27,28,29,30,31,32, 34, 35, 39]
Plasma	1 [33]
Serum and plasma	1 [36]
Serum and pleural effusion	1[37]
Serum and saliva	1 [38]
Storage temperature (°C)
− 80	9 [26,27,28,29,30, 32, 34, 35, 38]
− 20	6 [22,23,24,25, 37, 39]
− 70	1 [31]
− 85	1 [33]
− 20; − 70	1 [36]
Patient status/disease
Breast cancer	14 [22,23,24,25,26,27,28,29, 31,32,33,34,35, 38]
Pleural effusion	1 [37]
Adnexal masses and ovarian cancer	1 [30]
Pregnancy	1 [39]
Multiple disease	1 [36]
Measurement unit
U/mL	15 [22,23,24,25,26,27,28,29,30, 32,33,34, 36, 38, 39]
ng/mL	2 [35, 37]
U/L	1 [31]
Kit or instrument
CLEIA	4 [27, 28, 34, 35]
EIA	3 [25, 29, 39]
ELISA	3 [22, 37, 38]
HumaReader Plus made by HUMAN GmbH	2 [23, 24]
MEIA	2 [30, 31]
RIA	2 [26, 32]
CMIA	1 [33])
Vista vs Advia Centaur	1 [36]

ISSN: 2046-4053