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Table 2 Characteristics of studies included in the systematic review

From: Detection of Fusobaterium nucleatum in feces and colorectal mucosa as a risk factor for colorectal cancer: a systematic review and meta-analysis

First author (year) Country Study design No. of CRC /C Age, mean (SD) Exclusion of subjects with history of antibiotic use (period) Exclusion of subjects with history of IBD diagnosis Specimen type Specimen collection time Detection method Study quality based on NOS scores
CRC C
Amitay (2017) [40] Germany NCC 46/231 66.9 (-) 62.1 (-) No Yes Feces Before colonoscopy 16S rRNA gene analysis Poor
Yu (2015) [41] China CC 42/52 --- --- Yes (last 6 months) Yes Feces and biopsy c Feces: non-reported
Biopsies: left tissues
454 FLX pyrosequencing and PCR Poor
Zhang (2018) [42] China CC 130/130 60.5 (9.8) 58.6 (8.9) Yes (last 6 months) Yes Feces Before colonoscopy 16S rDNA sequencing and PCR Good
Flanagan (2014) [43] Ireland CC 7/25 --- --- No Yes Feces --- qPCR Poor
Fukugaiti (2015) [44] Brazil CC 7/10 65.4 (1.1) 54.8 (1.3) Yes (unspecified) No Feces Before colonoscopy qPCR Poor
Suehiro (2017) [45] Japan CC 158/60 69 (-) 32 (-) No No Feces Prior to bowel preparation dd-PCR Poor
Rezasoltani (2018) [46] Iran CC 20/31 60.9 (13.5) 59.8 (17) Yes (last 6 months) Yes Feces Before colonoscopy qPCR Poor
Eklof (2017) [47] Sweden NCC 39/65 34 (-) 34 (-) No Yes (for C only) Feces Prior to bowel preparation qPCR Good
Guo (2018) [48] China CC 215/156 --- --- Yes (last 3 months) Yes Feces Time of diagnosis, before resection qPCR Poor
Liang (2017) [49]            
 Cohort I China CC 170/200 67.2 (11.6) 59.3 (5.8) Yes (last 3 months) No Feces Before or one month after colonoscopy qPCR Good
 Cohort II China 33/36 63.4 (9.6) 53.2 (12.2)
Mira-Pascual (2015) [50] Spain CC 7/9 71.1 (10.1) 52.6 (15.2) No No Feces and biopsyd ≥ 1 week before colonoscopy qPCR Good
Repass (2018) [51] USA CC 40/40 --- --- No No Biopsy During surgery qPCR Fair
Russo (2017) [52] Italy CC 10/10 --- --- Yes (last 3 months) No Feces 1 day before surgery for CRC and unspecified for C qPCR Poor
Vogtmann (2016) [53] USA CC 52/52 61.8 (-) 61.2 (-) No No Feces Prior to surgery or other treatment WGSS Good
Wang (2016) [54] China CC 10/10 54.8 (-) 54 (-) No No Feces --- PCR Good
Wang (2012) [55] China CC 46/56 60a 49a Yes (last 3 months) Yes (for C only) Feces Before surgery for CRC patients and unspecified for C qPCR Poor
Wong (2017) [56] China CC 104/102 66.9 (10.1) 57.1 (5.8) Yes (last 3 months) Yes Feces Prior to bowel preparation for colonoscopy qPCR Poor
Wu (2013) [57] China CC 19/20 58.3 (8.7) 53.2 (5.4) Yes (last 3 months) Yes (for C only) Feces --- qPCR Good
Xie (2017) [58] China CC 327/242 63.5 (10.2) 60.1 (8.4) Yes (last month) Nob Feces Prior to bowel preparation for surgery or endoscopy qPCR Good
Yu (2016) [59] China CC 93/20 59.25(-) --- No No Biopsy Colonoscopy or surgery FISH Poor
Yu (2017) [60] China CC 74/54 63 (50.7) 67 (34.9) Yes (last 3 months) Yes Feces Before or mostly after colonoscopy Metagenomic sequencing Poor
Tunsjo (2019) [61] Norway CC 23/22 70 (-) 57 (-) No No Feces and biopsy e Prior to bowel preparation or 1 week after colonoscopy qPCR Poor
Liu (2020) [62] China CC 53/45 52.4 (18.8) 53.7 (16.7) Yes (last month) Yes Feces Prior to bowel preparation for colonoscopy qPCR Good
Kashani (2020) [63] Iran CS 35/45 --- --- No No Biopsy Colonoscopy PCR Satisfactoryf
  1. No. number, CRC colorectal cancer cases, C controls (colorectal-adenoma- and CRC-free subjects), IBD inflammatory bowel disease, NCC nested case-control, CC case-control, CS cross sectional, qPCR quantitative polymerase chain reaction, NOS Newcastle-Ottawa scale, WGSS whole genome shotgun sequencing, dd-PCR droplet digital PCR, FISH fluorescent in situ hybridization
  2. --- Not reported
  3. aMedian
  4. bControls were those with normal or chronic inflamed colorectal mucosa
  5. cBiopsies were taken from only 31/42 CRC and 37/52 C
  6. dBiopsies were taken from 7/7 CRC and 5/9 C
  7. eBiopsies were taken from 21/23 CRC and 11/22 C
  8. fIn NOS adapted for CS studies, the study quality is based on the total score and rated as follow: very good (9–10 points), good (7–8 points), satisfactory (5–6 points), and unsatisfactory (0 to 4 points)