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Table 3 Subgroup analyses by RT-PCR assay type

From: Effectiveness of real-time polymerase chain reaction assay for the detection of Mycobacterium tuberculosis in pathological samples: a systematic review and meta-analysis

RT-PCR assay type Summary measure of test accuracya (95% CI) Test for heterogeneity
(χ 2) (df = 24) (I 2) p value
CobasTaqMan (n = 17; b19,814) AUC = 0.98
 Sensitivity 0.78 (0.76–0.80) 205.13 92.20% < 0.001
 Specificity 0.99 (0.99–0.99) 127.21 87.40% < 0.001
 Positive likelihood ratio (PLR) 41.59 (27.80–62.18) 98.14 83.70% < 0.001
 Negative likelihood ratio (NLR) 0.18 (0.13–0.23) 134.36 88.10% < 0.001
 Diagnostic odds ratio (DOR) 273.14 (181.45–411.17) 47.6 66.40% < 0.001
Light Cycler (n = 7; b1159) AUC = 0.97
 Sensitivity 0.85 (0.80–0.88) 61.71 90.30% < 0.001
 Specificity 0.99 (0.98–0.99) 10.09 40.50% 0.121
 Positive likelihood ratio (PLR) 21.65 (6.82–68.72) 17.45 65.60% 0.008
 Negative likelihood ratio (NLR) 0.17 (0.08–0.38) 63.77 90.60% < 0.001
 Diagnostic odds ratio (DOR) 150.52 (31.97–708.78) 20.35 70.50% < 0.002
Cepheid and others (n = 22; b14,839) AUC = 0.99
 Sensitivity 0.78 (0.77–0.80) 729.43 97.10% < 0.001
 Specificity 0.99 (0.99–0.99) 299.13 93.00% < 0.001
 Positive likelihood ratio (PLR) 38.50 (19.65–75.42) 234.62 91.00% < 0.001
 Negative likelihood ratio (NLR) 0.22 (0.15–0.33) 594.30 96.50% < 0.001
 Diagnostic odds ratio (DOR) 221.44 (94.94–516.51) 208.25 89.90% < 0.001
  1. χ 2 chi-squared, df degree of freedom, I 2 I-squared, n number of studies, CI confidence interval, AUC area under receiver operating characteristics curve
  2. aRandom-effects model
  3. bNumber of specimens
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Systematic Reviews

ISSN: 2046-4053