Table 1 Characteristics of the included studies
Author year [n] | Country | Total number of samples (N) | Reference test: culture | Index test RT-PCR | Target sequence | |
|---|---|---|---|---|---|---|
PTB | EPTB | |||||
Albuquerque, 2014 [24] | Brazil | 140 | – | LJ 7H9 | TaqMan | IS6110 gene |
Antonenka, 2013 [25] | Germany | 116 | – | MGIT LJ | TaqMan | rpoB as target sequence |
Armand, 2011 [26] | France | 70 | 47 | LJ BacT/Alert MP | TaqMan | IS6110 gene |
Barletta, 2014 [27] | Belgium | 112 | – | LJ | Light Cycler 480 Real-time PCR assay | IS6110 gene |
Bloemberg, 2013 [28] | Switzerland | 829 | 280 | 7H11 MGIT | COBAS TaqMan | 16S rRNA gene |
Causse, 2011 [23] | Spain | – | 340 | LJ 7H9 | COBAS TaqMan MTB | 16S rRNA gene |
Chadran, 2010 [29] | India | 72 | – | LJ MGIT 960 | COBAS TaqMan | IS6110 gene |
Chaidir, 2012 [30] | Indonesia | – | 207 | Ogawa egg medium MB/BacTalert | IS6110-PCR BioRad | IS6110 gene |
Chang, 2015 [31] | South Korea | 2859 | – | MGIT 960 3% Ogawa | AdvanSure TB/NTM Real-time PCR assay | IS6110 gene |
Chen, 2012 [32] | China | 178 | – | BACTEC™ MGIT™ 960 | Real-time PCR assay used the ABI Prism SDS 7000 | IS6110 gene |
Chitnis, 2010 [33] | India | – | 204 | LJ MGIT-BACTEC | Geno-Sen’s MTB complex Real-time PCR assay | 16S rRNA gene |
Cho, 2015 [34] | South Korea | 2384 | 626 | 2% Ogawa medium MGIT 960 | COBAS TaqMan MTB assay | IS6110 gene |
Choe, 2011 [35] | South Korea | – | 129 | 3% Ogawa | TaqMan | Real-time MTB PCR, targeting the senX3-regX3 intergenic region |
Choi, 2013 [36] | South Korea | 360 | 65 | MGIT 960 | COBAS TaqMan MTB assay | IS6110 gene |
Dayal, 2010 [37] | India | – | 47 | BacT/Alert | Real-time PCR targeting 16SrRNA using Light Cycler RNA amplification syber green 1 kit (Roche Applied Biosciences, Germany) | 16S rRNA gene |
El Khechine, 2009 [38] | France | – | 134 | BACTEC 9000 MB LJ | Not specified | IS6110 gene |
Feizabadi, 2012 [39] | Iran | 247 | – | LJ | TaqMan | Cytochrome P450 Cyp 141 gene |
Friedrich, 2011 [40] | South Africa | – | 25 | MGIT 960 | Xpert | rpoB probe |
Gous, 2012 [41] | South Africa | – | 39 | BACTEC 9000 MB LJ | Light Cycler mycobacterium detection assay (LCTB) |  |
Hillemann, 2011 [42] | Germany | – | 521 | MGIT 960 LJ | GenoType MTBC and CM/AS assays (Hain Lifescience) | rpoB probe |
Hofmann-Thiel, 2016 [43] | Germany | 608 | 107 | MGIT LJ | Abbott Real-time MTB | Antigen b (PAB) and the multicopy insertion element IS6110 gene |
Huh, 2015 [44] | South Korea | 6852 | – | MGIT 960 LJ | COBAS TaqMan | 16S rRNA gene |
In, 2014 [45] | South Korea | 247 | – | BACTEC MGIT 960 LJ | Ultrafast NBS LabChip G2–3 (NanoBioSys) |  |
Jönsson, 2015 [46] | Sweden | 2388 | 1005 | MGIT 960 LJ | COBAS TaqMan | 16S rRNA gene |
Kheawon, 2012 [47] | Thailand | 430 | – | LJ medium | Commercial PCR Kits Amplicor | IS6110 gene and MPB64 gene |
Kim, 2011 [48] | South Korea | 96 | 310 | Solid culture | Ultrafast NBS LabChip G2–3 system (NanoBioSys) | 61 genomic DNA (gDNA) samples of MTB |
Lee, 2011 [49] | South Korea | 99 | – | 3% Ogawa | AdvanSure TB/NTM Real-time PCR assay | IS6110 gene |
Lee, 2010 [50] | South Korea | – | 143 | 3% Ogawa | Light Cycler 2.0 | senX3-regX3 intergenic region |
Lee, 2013 [51] | Taiwan | 587 | – | BACTEC MGIT 960 LJ | COBAS TaqMan MTB assay | IS6110 gene |
Lim, 2014 [52] | South Korea | 1167 | Â | Â | COBAS TaqMan MTB assay | Â |
Linasmita, 2012 [53] | Thailand | – | 73 | MGIT 960 | COBAS TaqMan MTB assay | 16S ribosomal RNA gene of M. tuberculosis |
Lira, 2013 [54] | Brazil | 165 | – | LJ | ABI Prism 7500 Sequence Detection System (Applied Biosystems) using TaqMan-specific probe | IS6110 gene |
Luo, 2010 [55] | USA | – | 70 | Culture-based assay (type not stated) | SmartCycler II instrument | IS6110 gene |
Malhotra, 2012 [56] | India | – | 555 | 7H9 | COBAS Taqman | IS6110 gene |
Mangat, 2016 [57] | India | 74 | – | MGIT 960 LJ | Roche Light Cycler 480 Real-time PCR system | 123 bp fragment of insertion element IS6110 sequence |
Miller, 2011 [58] | North Carolina, USA | 89 | 23 | 7H9 LJ | Xpert | Laboratory-developed test targeting IS6110 gene |
Moure, 2012 [59] | Spain | – | 149 | MGIT 960 LJ | GX assay |  |
Park, 2013 [60] | South Korea | 320 | – | MGIT 960 3% Ogawa | COBAS TaqMan | IS6110 gene |
Pinhata, 2015 [61] | Brazil | 715 | – | MGIT 960 Ogawa–Kudoh slant | Roche Light Cycler 480 II system | mpt64 gene |
Rachow, 2011 [11] | Tanzania | 292 | – | Both liquid and solid (type not stated) | Cepheid Xpert MTB/RIF assay | rpoB as target gene |
Rosso, 2011 [62] | Brazil | – | 158 | LJ | ABI Prism 7500 system (Applied Biosystems) | IS6110 gene |
Sethi, 2012 [63] | India | 50 | 22 | MGIT 960 LJ | In-house mpt64 Real-time PCR | mpt64 gene |
Sharma, 2015 [64] | India | 1480 | – | MGIT 960 LJ | ABI prism 3130xl genetic analyser (Applied Biosystems) | 81-bp rpoB gene |
Tortoli, 2012 [65] | Italy | 4340 | 1727 | MGIT 960 LJ | Cepheid Xpert MTB/RIF assay | rpoB as target gene |
Wang, 2013 [66] | China | 30 | – | Bact/Alert 3D | Light Cycler 480 (Roche) |  |
Yang, 2011 [67] | Taiwan | 1093 | – | MGIT 960 LJ 7H11 | COBAS TaqMan MTB assay | IS6110 gene |