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Table 1 Characteristics of the included studies

From: Paediatric tuberculosis diagnosis using Mycobacterium tuberculosis real-time polymerase chain reaction assay: a systematic review and meta-analysis

Author year

(n)

Country

Study design

Total number of samples (N)

Reference test:

culture

Index test:

RT-PCR

Target sequence

PTB

EPTB

Bates et al. (2013) [36]

Zambia-(L)

Prospective-descriptive

142

 

Liquid culture (MGIT)

RT-PCR Xpert MTB/RIF

rpoB probe

Bates et al. (2013) [36]

Zambia-(L)

Prospective-descriptive

 

788

Liquid culture (MGIT)

RT-PCR Xpert MTB/RIF

rpoB probe

Chipinduro et al. (2017) [37]

Zimbabwe-(L)

A cross-sectional

 

222 (stool)

LJ

RT-PCR Xpert MTB/RIF

rpoB probe

El Khechine et al. (2009)

[38]

France-(U)

Diagnostic case-control

–

134

BACTEC 9000 MB

LJ

RT-PCR(MX3000)

IS6110 gene

Gous et al. (2015) [39]

South Africa-(U)

Prospective

345

–

Liquid culture (MGIT)

RT-PCR Xpert MTB/RIF

rpoB probe

LaCourse et al. (2014) [40]

Malawi-(L)

Cross-sectional study

300

–

Bactec MGIT, BD)

RT-PCR Xpert MTB/RIF

rpoB probe

Memon et al. (2018) [41]

India-(L)

Diagnostic accuracy study

–

100

Bactec MGIT 960

RT-PCR Xpert MTB/RIF

rpoB probe

Mesman et al. (2019) [42]

Peru-(U)

Cohort study

 

259(stool)

BACTEC 9000 MB

LJ

TruTip Mtb DNA

IS6110 real-time PCR

Nhu et al. (2013) [43]

Vietnam-(L)

Prospective

 

96

MGIT, Becton Dickinson)

RT-PCR Xpert MTB/RIF

rpoB probe

Nicol et al. (2011) [44]

South Africa-(U)

Prospective-descriptive

452

–

Liquid culture

RT-PCR Xpert MTB/RIF

rpoB probe

Nicol et al. (2013) [45]

South Africa-(U)

Prospective

–

115

Bactec MGIT 960

RT-PCR Xpert MTB/RIF

rpoB probe

Nicol et al. (2018) [46]

South Africa-(U)

Cohort study

367

–

MGIT, Becton Dickinson)

RT-PCR Xpert MTB/RIF

rpoB probe

Oberhelman et al. (2010) [47]

Peru-(U)

Prospective case-control study

 

218 (stool, GA, etc.)

LJ culture

hemi-nested IS6110 PCR

IS6110 PCR

Qing-Qin Yin et al. (2014) [48]

China-(U)

Prospective

255

 

Solid (LJ) and Liquid culture (Bactec MGIT 960

RT-PCR Xpert MTB/RIF

rpoB probe

Rachow et al. (2012) [49]

Tanzania-(L)

Prospective cohort study

164

–

Solid (LJ) and Liquid culture (Bactec MGIT 960

RT-PCR Xpert MTB/RIF

rpoB probe

Sekadde et al. (2013) [50]

Uganda-(L)

Cross-sectional diagnostic study

235

–

Solid (LJ) and Liquid culture (Bactec MGIT 960

RT-PCR Xpert MTB/RIF

rpoB probe

Walters et al. (2017) [51]

South Africa-(U)

Prospective

 

379 (stool)

BACTEC 9000 MB

RT-PCR Xpert MTB/RIF

rpoB probe

Wang et al. (2013) [52]

China-(U)

Retrospective

30

–

Bact/Alert 3D

LightCycler® 480 (Roche)

 

Wolf et al. (2008) [53]

Peru-(U)

Diagnostic accuracy study

–

16 (6+) (stool)

 

hemi-nested IS6110 PCR

IS6110 PCR

Zar et al. (2012) [54]

South Africa-(U)

Prospective

535

–

Liquid culture (MGIT)

RT-PCR Xpert MTB/RIF

rpoB probe

Zar et al. (2013) [55]

South Africa-(U)

Prospective study

384

–

Bactec MGIT 960

RT-PCR Xpert MTB/RIF

rpoB probe

  1. Key: LJ Löwenstein-Jensen, Middlebrook 7H9 broth liquid growth medium, Middlebrook 7H11 Solid medium, MGIT mycobacterium growth indicator tube, PTB pulmonary TB, EPTB extra-pulmonary TB, n reference list number, L lower- and middle-income countries, U upper middle-income countries