- Item 1, species; Item 2, using appropriate tests; Item 3, severity of injury; Item 4, level of injury; Item 5, age/weight; Item 6, number of animals per group; Item 7, designation of strain; Item 8, definition of control; Item 9, description of statistical analysis; Item 10, regulation and ethics; Item 11, bladder expression; Item 12, blindness of assessor; Item 13, genetic background; Item 14, method of allocation to treatments; Item 15, attrition