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Table 4 Frequency of presence of F. nucleatum in CRC and controls’ specimens and odds ratio for the association between F. nucleatum and colorectal cancer

From: Detection of Fusobaterium nucleatum in feces and colorectal mucosa as a risk factor for colorectal cancer: a systematic review and meta-analysis

First author (year) Specimen type (no. of specimens CRC/C) Definition of specimen positive to F. nucleatum Prevalence of F. nucleatum (+) OR [95% CI] Adjusted OR [95% CI]
C (%) CRC (%) p
Amitay (2017) [40] Feces (46/231) F. nucleatum is detected 20 51 < 0.001 4.16 [2.15–8.07] ---
Fukugaiti (2015) [44] Feces (7/10) F. nucleatum is detected 90 100 --- 2.37 [0.08–66.88] ---
Liang (2017) [49] Coh I Feces (170/200) F. nucleatum is detected 72 98.2 < 0.0001 21.22 [6.57–68.5] ---
Mira-Pascual (2015) [50] Feces (7/9) F. nucleatum is detected 22.2 85 --- 19.86 [1.47–268.17] ---
Biopsy (7/5) F. nucleatum is detected 0 26,6 --- 4.6 [0.18–121.18] ---
Vogtmann (2016) [53] Feces (52/52) F. nucleatum is detected 26.9 63.5 0.0002 4.72 [2.05–10.85] ---
Wang (2016) [54] Feces (10/10) F. nucleatum is detected 10 60 --- 13.5 [1.2–152.21] ---
Wu (2013) [57] Feces (19/20) F. nucleatum is detected 0 68.4 --- 85.15 [4.42–1638.85] ---
Yu (2017) [60] Feces (74/54) F. nucleatum is detected 3.7 52.7 7.53E-08 29 [6.57–128] ---
Kashani (2020) [63] Biopsy (35/45) F. nucleatum is detected 24 68 0.0001 6.74 [2.5–18.07] ---
Repass (2018) [51] Biopsy (40/40) The product is amplified in the qPCR reaction 5 40 --- 12.67 [2.67–60.05] ---
Yu (2016) [59] Biopsy (93/20) Average number of bacteria per field ≥ 5, visualized by FISH technique Invasive 20, in biofilms 10 Invasive 65.9a, in biofilms 48.4a < 0.05 7.73 [2.38–25.07] e ---
Tunsjo (2019) [61] Feces (23/22) F. nucleatum detected with ΔCq values < 12 0 35 --- 24.9 [1.33–463.72] ---
Biopsy (21/11) F. nucleatum is detected 18 52 --- 4.93 [0.85–28.67] ---
Suehiro (2017) [45] Feces (158/60) F. nucleatum detected at a higher level based on cutoff value (> 260 copies )b 10 54 --- 10.57 [4.3–25.98] ---
Eklof (2017) [47] Feces (39/65) F. nucleatum detected at a higher level based on cutoff value (2−ΔCq > 0.00026c2−12 )c 24.3 69.2 --- 7 [2.89–16.96] ---
Xie (2017) [58] Feces (327/242) F. nucleatum detected at a higher level based on cutoff value (value is not reported) d --- --- --- 4.31 [2.96–6.28] 4.28 f [2.27–8.09]
  1. no. number, CRC colorectal cancer cases, C controls (colorectal-adenoma- and CRC-free subjects), OR odds ratio
  2. ---Not reported
  3. aThe value was calculated as the average of reported prevalence in proximal and distal separately
  4. b260 copies of F. nucleatum was the best cutoff point to discriminate between HC and CRC in receiver operating characteristic (ROC) analysis resulting in estimated sensitivity of 54% and estimated specificity of 90%, and the area under the ROC curve was 0.75
  5. cThe cutoff value of 0.00026 (= 2−ΔCq = 2−12) gave the most reliable analysis for detecting cancer in the study patients, with estimated specificity of 76.9%
  6. dROC curve was used to evaluate the diagnostic value of bacterial candidates in distinguishing CRC and controls and to determine the best cutoff values that maximized the Youden index
  7. eOR was calculated based on prevalence of invasive F. nucleatum (in tissues) as its detection was higher than within biofilms
  8. fOR adjusted for age, gender, FIT test, two additional bacteria markers, history of diabetes, and high blood pressure