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Table 3 Studies comparing F. nucleatum load in colorectal specimens of colorectal cancer cases and controls

From: Detection of Fusobaterium nucleatum in feces and colorectal mucosa as a risk factor for colorectal cancer: a systematic review and meta-analysis

First author (year) Specimen type (No. of specimens CRC/C) F. nucleatum quantification measures reported Statistics reported F. nucleatum load in CRC compared to C
C CRC p
Relative abundance
 Zhang (2018) [42] Feces (130/130) Relative contribution to total bacteria (%) Mean 0.001 0.17 < 0.001
 Vogtmann (2016) [53] Feces (52/53) Relative contribution to total bacteria (%) Mean 0.003 0.08 0.043
 Wang (2012) [55] Feces (46/56) Relative contribution to total bacteria (%) Mean 0.002 0.061 0.005
Absolute quantification
 Yu (2017) [60] Feces (74/54) Bacteria counts Rank mean 45.09 IMG
40.32 MLG
54.62 OTU
78.66 IMG
82.14 MLG
71.71 OTU
< 0.001
 Suehiro (2017) [45] Feces (158/60) Absolute copy number of F. nucleatum Median (min–max) 17.5 (0–5793) 317 (0–17,343) < 0.0001
 Fukugaiti (2015) [44] Feces (7/10) Log no. copies of F. nucleatum/gram Mean ± SD (min-max) 4.0 ± 1.5 (1.0–6.4) 6.2 ± 1.5 (3.5–8.0) 0.01
 Mira-Pascual (2015) [50] Feces (6/10) Log no. gene copies of F. nucleatum/mg Median (IQR) 4.16 (3.47–4.85) 4.70 (3.85–5.15) ---
 Rezasoltani (2018) [46] Feces (20/31) Cq value (Quantity of F. nucleatum = 10 (\( \frac{Cq-b}{m} \) )) Mean (SD) 29.16 (3.31) 17.74 (3.59) < 0.05
 Liu (2020) [62] Feces (53/45) Log 10 copies of F. nucleatum ∕ gram Mean (SD) ≈3.5 (1) ≈6 (1) < 0.01
Relative quantification
 Russo (2017) [52] Feces (10/10) Ratio: \( \frac{Cq\kern0.5em F. nucleatum\kern0.75em }{Cq\ total\ bacteria} \) Mean ≈2.25 ≈1.8 > 0.05
 Yu (2015) [41] Feces (42/52) ΔCq value Median [IQR] ≈26 [2] ≈19 [2] < 0.001
Biopsy (31/37) ≈27 [2] ≈18 [3] < 0.001
 Flanagan (2014) [43] Feces (7 /25) 2−ΔCq value Median 2−21 2−15 0.02
 Liang (2017) [49] Coh I Feces (170/200) 2−ΔCq value Median ≈2−17 ≈2−5 < 0.0001
 Liang (2017) [49] Coh II Feces (33/36) 2−ΔCq value Median ≈2−16 ≈2−13 0.012
 Eklof (2017) [47] Feces (39/66) Log 2−ΔCq Median [IQR] ≈− 10 [2] ≈− 7 [5] < 0.001
 Guo (2018) [48] Feces (215/156) Log 2−ΔCq Mean ≈−4 ≈− 3 < 0.0001
 Wong (2017) [56] Feces (104/102) Log 2−ΔCq (fold change from HC to CRC) Mean ≈−7 ≈−5 (132) < 0.001
 Xie (2017) [58] Feces (327/242) Log2 RQ, based on ΔCq (Fold change from HC to CRC) Mean ≈−20 ≈− 16 for early stage and −18 for advanced stage (5.12) 0.006
< 0.001
 Tunsjo (2019) [61] Feces (23/22) 2−ΔCq value (fold change from HC to CRC) Median ≈2−7 ≈2−5
(66)
0.0073
  1. CRC colorectal cancer cases, C controls (colorectal-adenoma- and CRC-free CRC and C subjects), SD standard deviation, Coh cohort, OTUs operational taxonomic units, MLGs metagenomic linkage groups, IMG integrated microbial genome, Cq quantification cycle in qPCR, ΔCq the average Cq value of F. nucleatum—the average Cq value of total bacteria (or reference gene), min minimum, max maximum
  2. ---Not reported
  3. A value read from a graph in the study material