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Table 3 Data to be extracted from each study

From: Do wastewater treatment plants increase antibiotic resistant bacteria or genes in the environment? Protocol for a systematic review

Category

Parameter to extract

Descriptive characteristics of the study

• Location of the study

• Study design

Type of sample, source and outcome

• Environmental media or biological sample type tested

• Location of the WWTP where the sample(s) was (were) taken

• Quantity of samples

• Bacterial species and/or genes analyzed

• Microbiological method used

Type of exposure

• Categorical: Downstream, downwind or down-gradient

• Continuous: Distance to the WWTP; distance to the Comparator

Type of comparator

• Categorical: Upstream, upwind or up-gradient

• Continuous: Distance to the WWTP; distance to the exposure site?

Sample size

• If exposure is categorical: Sample size of each group

• If exposure is continuous: Total sample size

If statistical modelling was used

• Type of model

• Effect measure (extracted or computed if there is enough raw data reported)

• Measure of variability

p value

Confounding control

• Methods to account for confounding or account for clustering data

Measure of resistance

• Proportional: Is denominator present? Was the denominator stable across sampling sites?

• Absolute