Author | Year | Assessed | Method | Increased in ME/CFS vs. HC (p value) | Decreased in ME/CFS vs. HC (p value) | No significance |
---|---|---|---|---|---|---|
Brenu et al. [42] | 2010 | NK cell phenotypes | Flow cytometry | CD56Bright (p < 0.05) | CD56Dim | |
NK cell cytotoxicity | K562% lysis (p < 0.05) | |||||
Brenu et al. [2] | 2011 | NK cell phenotypes | Flow cytometry | CD56Bright (p < 0.001) | CD56Dim | |
NK cell cytotoxicity | K562% lysis (p < 0.05) | |||||
Brenu et al. [43] | 2012 | NK cell phenotypes | Flow cytometry | CD56Bright (p = 0.02)a | CD56Dim | |
NK cell cytotoxicity | K562% lysis (p < 0.05)a | |||||
Brenu et al. [14] | 2013 | NK cell phenotypes | Flow cytometry | CD56Bright, CD56Dim KIR, NKG2D | ||
NK cell cytotoxicity | K562% (p < 0.05) | |||||
NK cell degranulation | CD107a (p < 0.05) | |||||
NK cell lytic proteins | Granzyme B (p < 0.05) | Perforin, Granzyme A | ||||
NK cell cytokine production | IFN-y (p < 0.05) | |||||
Curriu et al. [48] | 2013 | NK cell phenotypes | Flow cytometry | CD69 (p = 0.0004, p < 0.01)b NKp46 (p < 0.001), (p = 0.0023)b | CD57 (p = 0.052) (MFI) CD25CD56Bright (p = 0.0012) CD25CD56Dim (p = 0.0494) | CD56Bright, CD56Dim CD57 (p = 0.1942) |
NK cell cytotoxicity | K562% lysis | |||||
Fletcher et al. [45] | 2010 | NK cell cytotoxicity | 51Cr release | 51Cr (p < 0.000) | ||
Hardcastle et al. [46] | 2015 | NK cell phenotype | Flow cytometry | KIR3DL1/DL2 (p < 0.004)a KIR2DL1 (p = 0.011)a KIR2DL2/DL3 (0.049)a KIR2DS4 (0.023)a CD18+CD11c− (p = 0.036) CD18+CD2− (p = 0.009) SLAM (p = 0.046) | CD56BrightNKG2D (p = 0.014) KIR2DL2/DL3 (p = 0.045)a NKp46 (p = 0.021) | |
NK cell lytic proteins | Perforin, granzyme A, granzyme B | |||||
Huth et al. [21] | 2014 | NK cell phenotype | Flow cytometry | CD56BrightCD2+CD18+ (p < 0.01) CD56DimCD18+CD11a+CD11c+ (p < 0.05) | CD56DimCD2+CD18+ CD18+CD11a+CD11b+CD11c+ | |
NK cell degranulation | CD107a/b | |||||
NK cell lytic proteins | CD56Dim Granzyme B (p < 0.05) | Granzyme A, Perforin | ||||
Huth et al. [16] | 2016 | NK cell MAPK phosphorylation | Flow cytometry | MEK1/2 (p < 0.05) P38 (p < 0.05) | ERK1/2 (p < 0.05) | MEK1/2 P38 |
NK cell cytotoxicity | K562% lysis | |||||
NK cell degranulation | CD107a/b | |||||
NK cell lytic proteins | Perforin, Granzyme A, granzyme B | |||||
NK cell phenotype | CD56Bright, CD56Dim | |||||
NK cell cytokines | IFN-γ TNF-α GM-CSF | |||||
Maher et al. [20] | 2005 | NK cell phenotype | Flow cytometry | CD56+CD3− (p = 0.04) | ||
NK cell cytotoxicity | 51Cr release | 51Cr (p = 0.001) | ||||
NK cell lytic proteins | Flow cytometry | Perforin (p = 0.01) | ||||
Marshall-Gradisnik et al. [44] | 2016 | NK cell cytotoxicity | Flow cytometry | K562% lysis (p < 0.05). | ||
Nguyen et al. [49] | 2016 | NK cell phenotype | Flow cytometry | TRPM3+ expression in CD56Bright (p < 0.05) | TRPM3+ expression in CD56Dim | |
NK cell Ca2+ influx | CD56Bright (p < 0.05)c | |||||
Nguyen et al. [5] | 2017 | NK cell phenotype | Flow cytometry | CD56DimTRPM3+ (p < 0.01)c | TRPM3+ expression in CD56Bright (p < 0.05) CD69 (p < 0.05)c | CD69 |
NK cell degranulation | CD107a | |||||
NK cell Ca2+ influx | CD56Bright (p < 0.05)c | CD56Dim | ||||
NK cell cytotoxicity | K562% lysis (p < 0.05)c | |||||
Rivas et al. [51] | 2018 | NK cell phenotype | Flow cytometry | CD69 (p = 0.0011) CD56Bright (p = 0.0075) | NKG2C (p < 0.0001) | |
Stewart et al. [50] | 2003 | NK cell phenotype | Flow cytometry | CD56BrightCD8+ (p = 0.04) CD56BrightCD8− (p = 0.05)d | ||
Theorell et al. [47] | 2017 | NK cell phenotype | Flow cytometry | CD56Dim HLA-DR (p = 0.002)d | ||
NK cell cytotoxicity | 51Cr release | K562% lysis IFN-γ inhibition (p = 0.004, 0.009)c | ||||
NK cell lytic proteins | Flow cytometry | Perforin, Granzyme A/B | ||||
NK cell cytokines | CD56Dim IFNγ (p = 0.009)c | |||||
NK cell degranulation | CD107a |