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Table 4 Subgroup analyses by RT-PCR assay target sequence gene

From: Effectiveness of real-time polymerase chain reaction assay for the detection of Mycobacterium tuberculosis in pathological samples: a systematic review and meta-analysis

RT-PCR assay genes Summary measure of test accuracya (95% CI) Test for heterogeneity
(χ 2) (df = 24) (I 2) p value
IS6110 (n = 22; b12,004) AUC = 0.99
 Sensitivity 0.79 (0.77–0.81) 470.30 95.50% < 0.001
 Specificity 0.98 (0.98–0.98) 150.16 86.00% < 0.001
 Positive likelihood ratio (PLR) 31.76 (20.12–50.13) 134.64 84.40% < 0.001
 Negative likelihood ratio (NLR) 0.17 (0.12–0.24) 356.22 94.10% < 0.001
 Diagnostic odds ratio (DOR) 243.69 (127.07–437.37) 141.04 85.10% < 0.001
16S rRNA (n = 7; b12,074) AUC = 0.97
 Sensitivity 0.69 (0.66–0.72) 45.85 86.90% < 0.001
 Specificity 0.99 (0.99–0.99) 32.10 81.30% < 0.001
 Positive likelihood ratio (PLR) 67.64 (36.40–125.70) 34.60 82.50% < 0.001
 Negative likelihood ratio (NLR) 0.29 (0.24–0.36) 19.87 69.8% 0.003
 Diagnostic odds ratio (DOR) 287.19 (193.85–425.46) 9.12 34.20% 0.167
Other genes (n = 17; b11,870) AUC = 0.98
 Sensitivity 0.82 (0.80–0.84) 413.02 96.10% < 0.001
 Specificity 0.99 (0.99–0.99) 173.35 90.80% < 0.001
 Positive likelihood ratio (PLR) 42.48 (20.66–87.36) 123.92 87.10% < 0.001
 Negative likelihood ratio (NLR) 0.22 (0.13–0.37) 498.03 96.80% < 0.001
 Diagnostic odds ratio (DOR) 234.56 (86.01–639.63) 125.08 87.20% < 0.001
  1. χ 2 chi-squared, df degree of freedom, I 2 I-squared, n number of studies, CI confidence interval, AUC area under receiver operating characteristics curve, IS6110 Mycobacterium tuberculosis complex-specific insertion sequence, 16S rRNA 16S ribosomal RNA gene of MTB, Other genes rpoB as target sequence, mpt64 gene, 81-bp rpoB gene, senX3-regX3 intergenic region, 61 genomic DNA (gDNA) samples of MTB, cytochrome P450 Cyp 141 gene
  2. aRandom-effects model
  3. bNumber of specimen