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Table 1 Characteristics of the included studies

From: Effectiveness of real-time polymerase chain reaction assay for the detection of Mycobacterium tuberculosis in pathological samples: a systematic review and meta-analysis

Author year [n] Country Total number of samples (N) Reference test: culture Index test RT-PCR Target sequence
PTB EPTB
Albuquerque, 2014 [24] Brazil 140 – LJ 7H9 TaqMan IS6110 gene
Antonenka, 2013 [25] Germany 116 – MGIT LJ TaqMan rpoB as target sequence
Armand, 2011 [26] France 70 47 LJ BacT/Alert MP TaqMan IS6110 gene
Barletta, 2014 [27] Belgium 112 – LJ Light Cycler 480 Real-time PCR assay IS6110 gene
Bloemberg, 2013 [28] Switzerland 829 280 7H11 MGIT COBAS TaqMan 16S rRNA gene
Causse, 2011 [23] Spain – 340 LJ 7H9 COBAS TaqMan MTB 16S rRNA gene
Chadran, 2010 [29] India 72 – LJ MGIT 960 COBAS TaqMan IS6110 gene
Chaidir, 2012 [30] Indonesia – 207 Ogawa egg medium MB/BacTalert IS6110-PCR BioRad IS6110 gene
Chang, 2015 [31] South Korea 2859 – MGIT 960 3% Ogawa AdvanSure TB/NTM Real-time PCR assay IS6110 gene
Chen, 2012 [32] China 178 – BACTEC™ MGIT™ 960 Real-time PCR assay used the ABI Prism SDS 7000 IS6110 gene
Chitnis, 2010 [33] India – 204 LJ MGIT-BACTEC Geno-Sen’s MTB complex Real-time PCR assay 16S rRNA gene
Cho, 2015 [34] South Korea 2384 626 2% Ogawa medium MGIT 960 COBAS TaqMan MTB assay IS6110 gene
Choe, 2011 [35] South Korea – 129 3% Ogawa TaqMan Real-time MTB PCR, targeting the senX3-regX3 intergenic region
Choi, 2013 [36] South Korea 360 65 MGIT 960 COBAS TaqMan MTB assay IS6110 gene
Dayal, 2010 [37] India – 47 BacT/Alert Real-time PCR targeting 16SrRNA using Light Cycler RNA amplification syber green 1 kit (Roche Applied Biosciences, Germany) 16S rRNA gene
El Khechine, 2009 [38] France – 134 BACTEC 9000 MB LJ Not specified IS6110 gene
Feizabadi, 2012 [39] Iran 247 – LJ TaqMan Cytochrome P450 Cyp 141 gene
Friedrich, 2011 [40] South Africa – 25 MGIT 960 Xpert rpoB probe
Gous, 2012 [41] South Africa – 39 BACTEC 9000 MB LJ Light Cycler mycobacterium detection assay (LCTB)  
Hillemann, 2011 [42] Germany – 521 MGIT 960 LJ GenoType MTBC and CM/AS assays (Hain Lifescience) rpoB probe
Hofmann-Thiel, 2016 [43] Germany 608 107 MGIT LJ Abbott Real-time MTB Antigen b (PAB) and the multicopy insertion element IS6110 gene
Huh, 2015 [44] South Korea 6852 – MGIT 960 LJ COBAS TaqMan 16S rRNA gene
In, 2014 [45] South Korea 247 – BACTEC MGIT 960 LJ Ultrafast NBS LabChip G2–3 (NanoBioSys)  
Jönsson, 2015 [46] Sweden 2388 1005 MGIT 960 LJ COBAS TaqMan 16S rRNA gene
Kheawon, 2012 [47] Thailand 430 – LJ medium Commercial PCR Kits Amplicor IS6110 gene and MPB64 gene
Kim, 2011 [48] South Korea 96 310 Solid culture Ultrafast NBS LabChip G2–3 system (NanoBioSys) 61 genomic DNA (gDNA) samples of MTB
Lee, 2011 [49] South Korea 99 – 3% Ogawa AdvanSure TB/NTM Real-time PCR assay IS6110 gene
Lee, 2010 [50] South Korea – 143 3% Ogawa Light Cycler 2.0 senX3-regX3 intergenic region
Lee, 2013 [51] Taiwan 587 – BACTEC MGIT 960 LJ COBAS TaqMan MTB assay IS6110 gene
Lim, 2014 [52] South Korea 1167    COBAS TaqMan MTB assay  
Linasmita, 2012 [53] Thailand – 73 MGIT 960 COBAS TaqMan MTB assay 16S ribosomal RNA gene of M. tuberculosis
Lira, 2013 [54] Brazil 165 – LJ ABI Prism 7500 Sequence Detection System (Applied Biosystems) using TaqMan-specific probe IS6110 gene
Luo, 2010 [55] USA – 70 Culture-based assay (type not stated) SmartCycler II instrument IS6110 gene
Malhotra, 2012 [56] India – 555 7H9 COBAS Taqman IS6110 gene
Mangat, 2016 [57] India 74 – MGIT 960 LJ Roche Light Cycler 480 Real-time PCR system 123 bp fragment of insertion element IS6110 sequence
Miller, 2011 [58] North Carolina, USA 89 23 7H9 LJ Xpert Laboratory-developed test targeting IS6110 gene
Moure, 2012 [59] Spain – 149 MGIT 960 LJ GX assay  
Park, 2013 [60] South Korea 320 – MGIT 960 3% Ogawa COBAS TaqMan IS6110 gene
Pinhata, 2015 [61] Brazil 715 – MGIT 960 Ogawa–Kudoh slant Roche Light Cycler 480 II system mpt64 gene
Rachow, 2011 [11] Tanzania 292 – Both liquid and solid (type not stated) Cepheid Xpert MTB/RIF assay rpoB as target gene
Rosso, 2011 [62] Brazil – 158 LJ ABI Prism 7500 system (Applied Biosystems) IS6110 gene
Sethi, 2012 [63] India 50 22 MGIT 960 LJ In-house mpt64 Real-time PCR mpt64 gene
Sharma, 2015 [64] India 1480 – MGIT 960 LJ ABI prism 3130xl genetic analyser (Applied Biosystems) 81-bp rpoB gene
Tortoli, 2012 [65] Italy 4340 1727 MGIT 960 LJ Cepheid Xpert MTB/RIF assay rpoB as target gene
Wang, 2013 [66] China 30 – Bact/Alert 3D Light Cycler 480 (Roche)  
Yang, 2011 [67] Taiwan 1093 – MGIT 960 LJ 7H11 COBAS TaqMan MTB assay IS6110 gene
  1. Key: LJ Löwenstein-Jensen, Middlebrook 7H9 Broth Liquid growth medium, Middlebrook 7H11 Solid medium, MGIT Mycobacterium Growth Indicator Tube, PTB Pulmonary TB, EPTB Extra-pulmonary TB, [n] reference list number
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Systematic Reviews

ISSN: 2046-4053