PCR-based specific techniques used for detecting the most important pathogens on strawberry: a systematic review

Table 1 Studies included in the systematic review of PCR techniques used for detecting of strawberry pathogens

Year	First author	Pathogen	PCR method	Sample preparation (long-term storage)	Origin of culture	Reference
1996	Sreenivasaprasad	CA	Conventional	NG	UK	[73]
1996	Roberts	XF	Conventional + nested	-70°C in 15% glycerol	US	[49]
1996	Pooler	XF	Multiplex	NG	US	[47]
1997	Bonants	PF	Nested	V8 oatmeal agar containing 50 ppm vancomycin/French bean agar at 4°C	Scotland + Netherlands	[45]
1997	Mahuku	XF	Nested	-70°C in 25% glycerol	Canada	[50]
1997	Zhang	XF	Conventional	NG	US	[74]
2002	Rigotti	BC	Conventional (Southern blot hybridization)	NG	Switzerland	[22]
2004	Stöger	XF	Conventional	NG	Austria	[16]
2004	Zimmermann	XF	Nested	-20°C in 30% glycerol	Germany	[48]
2004	Bonants	PF	Nested + real-time (TaqMan, Mol. Beacon) + PCR-ELISA	V8 agar at 11°C	Netherlands	[13]
2005	Suarez	BC	Real-time (TaqMan)	Frozen plastic bag at -20°C	UK	[21]
2006	Ioos	PF	Conventional	NG	France	[19]
2006	Drenth	PF	Conventional	Freeze-dried at -70°C	Australia	[72]
2007	Weller	XF	Real-time (TaqMan)	NG	UK	[51]
2008	Vandroemme	XF	Real-time (TaqMan)	NG	Belgium	[18]
2008	Turechek	XF	Real-time (TaqMan)	NG	US	[17]
2008	Pérez-Hernández	CA	Nested + conventional	NG	US	[29]
2008	Kuchta	VD	Conventional	Czapek-Dox Agar at 4°C	Poland	[46]
2009	Debode	CA	Real-time (TaqMan)	NG	Belgium	[27]
2009	Garrido	CA	Conventional + real-time (TaqMan)	Sterile water at 4°C	Spain + UK	[28]
2012	Bilodeau	VD	Multiplexed real-time (TaqMan)	NG	US	[33]
2013	Suga	FO	Multiplex	-80°C in 50% glycerol	Japan	[1]

XF Xanthomonas fragariae, PF Phytophthora fragariae, BC Botrytis cinerea, VD Verticillium dahliae, FO Fusarium oxysporum, CA Colletotrichum acutatum, NG not given.

ISSN: 2046-4053